You have constructed a standard curve from BSA protein for a Bradford Assay. The curve has 0, 1, 2, 3, and 4 ug protein in 50 ul water. You have an unknown protein with concentration somewhere between 1-5 mg/mL. What serial dilution series should you use to guarantee at least two dilutions of your unknown fall below 3 ug (linear portion) on the standard curve? For example, would 1/2 x 1/2 x 1/2 (3 2-fold dilutions) be sufficiently dilute that two of the dilutions would give values within the linear portion of the standard curve?
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
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