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What is your overall dilution factor if you complete 3 serial dilutions using a 100-fold dilution each time? (Show your work)
Introduction
Dilution is the process of making a solution weaker or less concentrated by adding more solvent (usually water). This results in a lower concentration of solute (the substance being dissolved) in the solution. The amount by which the solution is diluted is often expressed as a "dilution factor."
Step by step
Solved in 2 steps
- How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.Using 25 uL of a patient's serum, how much diluent would be needed to create a 1:2 dilution? ______ ulLabel the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.
- A.How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.how to calculate the experiment fow rate for this gel chromatography Experiment flow rate ? Flow rate 4.75mL/min
- how does the diameter of the column affect the flow rate of the elution in gel filtration chromatography?DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?Please calculate the denaturation percentage
- Using a P-1000 micropipette, you transfer 800 microliter of ddH2O to a beaker and measure its weight. It turns out to be only 780 milligram; what is the percent error of this micropipette?how did you get 104 as the dilution factor? I got 10-2 as the dilution factor for the CNFL plateIn your opinion and based on your knowledge, what is the most important component that will be tested using the reagent test strips?
