Genomic DNA was isolated from three individuals shown in the pedigree above. Primers were used to amplify DNA sequences at a particular locus using PCR and the DNA was resolved by gel electrophoresis. The result is presented in Figure I. What can we conclude from the gel patterns about which band corresponds to which allele

Biology: The Dynamic Science (MindTap Course List)
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Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications
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Genomic DNA was isolated from three individuals shown in the pedigree above. Primers were used to amplify DNA sequences at a particular locus using PCR and the DNA was resolved by gel electrophoresis. The result is presented in Figure I. What can we conclude from the gel patterns about which band corresponds to which allele (HINT: think dominant/recessive)? (3 sentences minimum)
III IV
V
VII OTVI
Figure 1: Family pedigree tracing a genetic
disease.
Individual Individual Individual
I
II
III
Origin
Larger Fragments
Smaller Fragments
Figure II: Image of a gel
electrophoresis of DNA fragments from
the same gene obtained from 3
individuals in the above pedigree.
Transcribed Image Text:III IV V VII OTVI Figure 1: Family pedigree tracing a genetic disease. Individual Individual Individual I II III Origin Larger Fragments Smaller Fragments Figure II: Image of a gel electrophoresis of DNA fragments from the same gene obtained from 3 individuals in the above pedigree.
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