How to find unknown gene and design primers present using whole genome sequence?

Q: What is linker scanning mutagenesis?

A: Answer: Introduction: Old-style approaches, containing linker-scanning mutagenesis includes…

Q: Why is genome database and annotation relevant to Genome sequencing?   Are there any examples?

A: Genome: the set of genes. Genome database: it is the collection of all information on the human…

Q: Which is a limitation of using massively parallel sequencing ?

A: It is a term used to describe the method of high-throughput DNA sequencing to determine the entire…

Q: What would be some major impediments to genetically modifyinghuman embryos with CRISPR?

A: Genome editing tools are those tools which help in making changes into the genome of an individual.…

Q: what are the purpose of sequence alignment? define local alignment and global alignment?

A: Alignments are an effective method for comparing similar DNA or protein sequences. DNA molecules are…

Q: Explain the Whole Genome Sequence Analysis ?

A: Deoxyribonucleic acid (DNA) is a double stranded helical genetic material containing thousands of…

Q: Explain Two strategies for assembling whole genome sequences.

A: All the genetic information of an organism is known as its genome. Genome assembly is defined as the…

Q: How to do linear Restriction Mapping of gene fragments, including a combination of three enzymes?

A: Bacteria contain restriction enzymes. To destroy viruses, bacteria use restriction enzymes, which…

Q: What is the principle of next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: How does precision help in genetic engineering and recombinant technology? How does accuracy help in…

A: genetic engineering and recombinant technology are modern geneticist tools which help to edit genes…

Q: How does a DNA microarray work?

A: Gene microarray technology enables to deposit many different DNA sequences on a small glass slide,…

Q: How are probes used to screen DNA libraries? Explain how a synthetic probe can be prepared when the…

A: A probe is an oligonucleotide stretch of DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) which…

Q: How accurate is whole genome sequencing?

A: The genome of an organism is defined as the whole heredity information encoded in the genetic…

Q: Describe step 1: hybridization of template and primer in automated sanger sequencing

A: Introduction  Sanger sequencing is also known as the “chain termination method”. It is a method for…

Q: How does gel electrophoresis separate different-sized DNA fragments?

A: Gel electrophoresis is a molecular biology technique used to separate macromolecules like DNA, RNA…

Q: What is hierarchical sequencing?

A: Basically there are two approaches for the sequencing of large complex genome. They are hierarchical…

Q: Who proposed shotgun sequencing?

A: The method used for the sequencing of random DNA strands is genetics, shotgun sequencing.  This is…

Q: What are gene microarrays? How are microarrays used?

A: The process of forming a single double-stranded nucleic acid molecule by combining two…

Q: How did the private corporation Celera Genomics approach the sequencing of the human genome? What…

A: Celera Genomics is a private biotechnology company that raced with the IHGSC to complete the…

Q: How to create DNA-sequence alignment ?

A: DNA-Sequence alignment can be defined as the way in which sequences of DNA samples are arranged for…

Q: How is recombinant DNA inserted into a genome using CRISPRediting?

A: The modern technology of genetics can be utilized to increase the genomes of various living…

Q: What is a cloning vector? Give two examples.

A: A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into…

Q: What types of cells are used for cloning rDNA?

A: rDNA is called recombinant DNA which contains DNA from multiple sources. Recombinant DNA is it's the…

Q: How is exome sequencing helps in identifying mutations?

A: Exome sequencing refers to the genomic technique in which the sequencing of all the protein-coding…

Q: What are the Strategies for genome sequencing?

A: The genetic material in the cells is present in the form of DNA. It contains the coded form of…

Q: Define the Single-cell sequencing (SCS) ?

A: Working of Single-cell deoxyribonucleic acid genome sequencing involves analytic one cell,…

Q: Briefly describe the steps or methods for studying genes.  Start with targeting a gene region and…

A: Gel electrophoresis is a strategy for investigating DNA dependent on its size (base-pair length). As…

Q: How to use a microarray for transcriptome analysis ?

A: Transcriptome is the complete set of the mRNA molecules expressed in an individual . It is termed as…

Q: what are similarities and differences between chain-termination and reversible terminator…

A: Gene sequencing  aids in the development of gene therapy, a form of treatment that replaces faulty…

Q: What does whole exome sequencing reveal?

A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…

Q: What is the principle behind to Sanger sequencing?

A: Sanger sequencing is a method of DNA sequencing, which is based on selective incorporation of…

Q: What are the two methods used for sequencing in Human genome project.

A: Answer- Human genome project was the mega project that aimed for sequencing of whole human genome.

Q: What does massively parallel sequencing mean?

A:

Q: What is genomic sequencing and what are the 3 primary ways of annotating a gene?

A: A genome sequence:It is the total nucleotides list that include A, C, G, and T for the DNA genomes…

Q: How are different variable region sequences generated?

A: Ans: The variable regions of the antibody have two chains heavy and light chains. They form a part…

Q: How to identify human genes during library screening ?

A: The term genomic library can be referred to as a collection of DNA clones that, in theory, should…

Q: How can libraries be used to locate a specific gene of interest?

A: Scientists use genetic engineering methods to create two different types of DNA libraries. These are…

Q: How is a genome-wide association study carried out?

A: Deoxyribonucleic acid (DNA) is a genetic material of most organisms. DNA contains the instructions…

Q: How can specific genes be retrieved from a DNA library?

A: Deoxyribonucleic acid or DNA is a molecule that contains the genetic code of the organisms. DNA…

Q: What will be the result if we resequence the same samples contributed to the reference genome and…

A: Many hereditary disorders may now be tested for through "genetic testing". Some diseases,…

Q: What are the main differences between bottom up/shotgun and top down proteomics strategies?

A: Proteomics studies plays an important role in biomarkers and drug targets. Mass spectrometry (MS) is…

Q: What is a microarray? How can it be used to obtain information about gene function?

A: A gene is a specific nucleotide sequence in RNA or DNA. It is generally located on a chromosome.…

Q: What is the results of extracting the DNA of a strawberry?

A: Ripe strawberries are an excellent source for extracting DNA because they are easy to pulverize and…

Q: Describe how lon torren method of sequencing works in detail.

A: Thermo Fisher Scientific's Ion Torrent technology introduces a completely new approach to Next…

Q: What key molecules are essential for Sanger sequencing?

A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.