Given a 0.300 M solution of anserine at its isoelectric point and ready access to 0.100 M HCl, 0.100 M NaOH and distilled water, describe the preparation of 1 L of 0.0300 M anserine buffered solution, pH 7.20. Volume of stock solution needed =  mL Volume of HCl needed to adjust the imidazole group =  mL Volume of HCl needed to titrate the amino group =  mL Total HCl needed =  mL

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Given a 0.300 M solution of anserine at its isoelectric point and ready access to 0.100 M HCl, 0.100 M NaOH and distilled water, describe the preparation of 1 L of 0.0300 M anserine buffered solution, pH 7.20.

Volume of stock solution needed =  mL

Volume of HCl needed to adjust the imidazole group =  mL

Volume of HCl needed to titrate the amino group =  mL

Total HCl needed =  mL

pi=8.27

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