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- Briefly describe in your own words what DNA barcoding is. Explain how PCR is used in this process.Draw your own PCR diagram to show the role of each component and relevance of each temperature shift during the PCR reaction. What’s happening at each of those temperatures and why is it important it changes?Discuss how the technique of PCR allows a scientist to quickly clone a particular piece of DNA.Be clear about the importance of 2 primers, Taq DNA polymerase, temp, and time. Explain howyou do a PCR – what is needed
- You are working in a molecular biology laboratory and are having challenges with your PCR. You decidet todouble-check the PCR protocol programmed into the thermal cycler and discover that the annealing temperature was programmed to be 65 °C instead of 50 °C, as you had intended. What effect would this mistake have on the PCR reaction? Suggest potential solutionspls solve last part PCR of this ques as per instructions needed only diagram with full explanation I'll give you many upvotesDraw your own series of labelled diagrams to describe the polymerase chain reaction (PCR) and its reagents in detail and give two examples of its use in clinical microbiology. Thank you!
- Quantitative PCR (qPCR) has one extra ingredient to quantify DNA. Describe its structure and explain how it changes during the reaction.Select all that apply: Which of these components must be added to a PCR reaction for it to produce a product?O DNA PrimersO Buffers- dNTPsO RNA PrimersO NTPSO PrimaseO Template DNAO Taq DNA PolymerasePolymerase Chain Reaction (PCR) works by exposing the reaction to a series of temperature changes. In 150 words or fewer, describe 1.) the different stages of a single PCR cycle, 2.) how many cycles of PCR your reactions will undergo, and 3.) why multiple cycles are necessary.
- Centrifugation is a common step in many molecular biology protocols. In your own words, explainhow it works.Explain why the PCR is unlikely to amplify contaminating bacterial DNA in a sample of human DNA.Explain how PCR could be used to pick a gene out of a complex genome and amplify it.Briefly explain the rationales of adding chemicals that can affect DNA stability in a polymerase chain reaction (PCR). Why DNA melting is required in PCR? Briefly explain how PCR can be used to detect DNA mutation.