2nd (middle) base1stbaseUC|A|G d3rdbaseUUU Phe UCU Ser UAU Tyr UGU CysUUC Phe UCC Ser UAC Tyr UGC CysUUUUA Leu UCA Ser UAA stop UGA stopAUUG Leu UCG Ser UAG stop UGG TrpGCUU Leu CCU ProCAU His CGU ArgCUC Leu CCC Pro CAC His CGC ArgCUA Leu CCA Pro CAA Gln CGA ArgCUG Leu CCG Pro CAG Gln CGG ArgUCAGCAUU IleAUC IleAUA IleACU Thr AAU Asn AGU SerACC Thr AAC AsnAAA LysGC SerAGA ArgAGG ArgАACA ThrAUG Met ACG Thr AAG LysAGGUU Val GCU Ala GAU Asp GGU GlyUGUC Val GCC Ala GAC Asp GGC GlyGGUA ValGAA Glu GGA GlyGCA AlaA.GUG Val GCG Ala GAG Glu GGG GlyG Which of the following best describes the complete sequence of steps occurring during evecycle of PCR?1- The primers hybridize to the target DNA.2-The mixture is heated to a high temperature to denature the double stranded target DNA3- Fresh DNA polymerase is added.4- DNA polymerase extends the primers to make a copy of the target DNA. E(2 Puan)2, 3, 43, 4, 22, 1, 43, 4, 1, 21, 3, 2, 4

PCR is an abbreviation for polymerase chain reaction which was developed by the biochemist Kary…

Which of the following best describes the complete sequence of steps occurring during eve cycle of PCR? 1- The primers hybridize to the target DNA. 2-The mixture is heated to a high temperature to denature the double stranded target DNA 3- Fresh DNA polymerase is added. 4- DNA polymerase extends the primers to make a copy of the target DNA. S (2 Puan)

Which of the following best describes the complete sequence of steps occurring during eve cycle of PCR? 1- The primers hybridize to the target DNA. 2-The mixture is heated to a high temperature to denature the double stranded target DNA 3- Fresh DNA polymerase is added. 4- DNA polymerase extends the primers to make a copy of the target DNA. S (2 Puan)

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 10QP: Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands...

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Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerase
Which of the following best describes the complete sequence of steps occurring during every cycle of PCR? I-The primers hybridize to the target DNA. II-The mixture is heated to a high temperature to denature the double stranded target DNA. III-Fresh DNA polymerase is added. IV-DNA polymerase extends the primers to make a copy of the target DNA. A) II, I, IV. B) I, III, II, IV. C) III, IV, I, II. D) III, IV, II. E) II, III, IV.
The PCR reaction contains deoxynucleotide triphosphates (dNTPs) in order to construct new DNA. There are four different dNTPs used in this reaction. Which answer below lists the four different dNTPs used in PCR? A) DNA polymerase, helicase, ligase, topoisomerase B) adenine, guanine, cytosine, thymine C) adenine, guanine,cytosine, uracil D) Alanine, guanine, cytosine, Theron one
Which of the following correctly matches the step of polymerase chain reaction (PCR) with its events? A. Extension - reaction is cooled and primers bind to complementary sequences on template DNA. B. Denaturation - high temperature separates the DNA C. Annealing - Taq polymerase extends the primers, synthesizing DNA
A student carries out PCR using the following steps: step 1: 94C for 1 minuteStep 2: 60C for 30seconds Step 3: 72C for 30 seconds The student is examining a 1000-bp DNA fragment as part of their research project. If the limit of detection of this molecule 9 x 108 molecules, what is the minimum number of PCR cycles you would have to run to detect PCR product generated from a single molecule of the template? 2. Two double-stranded fragments of DNA are exactly the same length. At 89 C, fragment A has completely denatured which means the two strands have separated. At that temperature, fragment B is still double-stranded. How might these fragments differ to result in different denaturation temperatures?
PCR (polymerase chain reaction) is an excellent method of generating copies of target DNA. If a single piece of double stranded DNA (dsDNA) is put into a PCR machine, how many dsDNA segments will there be after 3 rounds? A. 8 segments, with 2 original strands paired B. 16 segments, with 2 original strands on different segments C. 16 segments, with 2 original strands paired D. 8 segments, with 2 original strands on different segments
During PCR, the reaction mixture cycles through three temperatures (for example 94, 60, and 72 degrees Celsius) multiple times. What happens during the 94 degree part of a cycle? Group of answer choices Double-stranded DNA denatures and becomes single-stranded. DNA polymerase synthesizes daughter DNA molecules. An oligonucleotide primer anneals to a template DNA strand.
Put the steps of one PCR cycle in the correct order: The PCR reaction mixture is heated to about 70 degrees, which is the optimum temperature for the polymerase to build the new strands of DNA, starting at the 3' end of the primer. The PCR reaction mixture is heated to 95 degrees Celsius, which denatures the double stranded template DNA. The PCR reaction mixture is cooled to about 50-55 degrees, which allows the primers to find their complementary site on the template and "anneal" the
Explain why a positive control and negative control are included in PCR experiments. Explain the three steps involved in each cycle of polymerase chain reaction.Why is loading dye added to the DNA sample for gel electrophoresis? Explain the function of the following components in a PCR reaction:− Primer, dNTP, MgCl, Taq polymerase, buffer.
Match the following terms with their definitions and label each component of the PCR mixture in the diagram (use the letters A-D):I. DNA polymeraseII. PrimersIII. NucleotidesIV. Genomic DNA template A. DNA that contains the target sequence that will be replicated using PCR.B. An enzyme that copies the DNA sequence.C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated DNA sequence.D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.
Would it be possible to use human polymerase for the PCR reaction? a. No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur. b. No, because human polymerase cannot be extracted from cells to use in a lab setting. c. Yes, because we are using human DNA as the template DNA. d. Yes, because human polymerase can add bases to a template strand without a primer.
Most PCR reactions do not use the more expensive types of DNA polymerase, which have DNA proofreading. How might this be a problem in accurately copying specific DNA sequences of the target gene?