Glycogen was isolated from a liver sample. Sixty milligrams of the crude glycogen were then dissolved in 10.0 mL distilled water. From this stock solution, 0.50 mL aliquot was taken and transferred onto a new tube. To this sample, all the reagents for acid hydrolysis. were added giving a total volume of 10.00 mL. A 0.50 mL was then taken and diluted with 0.50 mL of distilled water. The glucose content from the completely hydrolyzed glycogen was measured using Nelson's assay giving an absorbance of 0.390. Standard solutions were prepared by diluting each aliquot of glucose solution to 1.00 mL and then subjecting each to Nelson's method. The following data were obtained from this experiment.
Glycogen was isolated from a liver sample. Sixty milligrams of the crude glycogen were then dissolved in 10.0 mL distilled water. From this stock solution, 0.50 mL aliquot was taken and transferred onto a new tube. To this sample, all the reagents for acid hydrolysis. were added giving a total volume of 10.00 mL. A 0.50 mL was then taken and diluted with 0.50 mL of distilled water. The glucose content from the completely hydrolyzed glycogen was measured using Nelson's assay giving an absorbance of 0.390. Standard solutions were prepared by diluting each aliquot of glucose solution to 1.00 mL and then subjecting each to Nelson's method. The following data were obtained from this experiment.
Chapter21: Heparin Infusion Calculations
Section: Chapter Questions
Problem 1.3P
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What is the dilution factor used for the glycogen isolate? Show complete solution.
Transcribed Image Text:Glycogen was isolated from a liver sample. Sixty milligrams of the crude glycogen were
then dissolved in 10.0 mL distilled water. From this stock solution, 0.50 mL aliquot was
taken and transferred onto a new tube. To this sample, all the reagents for acid hydrolysis
were added giving a total volume of 10.00 mL. A 0.50 mL was then taken and diluted with
0.50 mL of distilled water. The glucose content from the completely hydrolyzed glycogen
was measured using Nelson's assay giving an absorbance of 0.390. Standard solutions
were prepared by diluting each aliquot of glucose solution to 1.00 mL and then subjecting
each to Nelson's method. The following data were obtained from this experiment.
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