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- Is is best to observe slides of bacteria stained with the acid fast stain under high dry magnification. True or falseThe unknown observations are shown as a mixed stain, gram positive, and gram negative. The next images shows the chart of what we can choose from for the gram positive bacterial stain. We are allowed to choose 4 test or stains that we would like to use to preform to identify the unknown.Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.
- The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope at 10x magnification. Samples were stained with Lugols solution for better viewing. Differentiate these two imageAfter performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explain why, the result you would want to observe and the interpretation of the result.After performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explainwhy, the result you would want to observe and the interpretation of the result.
- In the image attached is a unknown bacteria lab. The gram stain result and growth result is already given to us and is shown. What other tests (1-2 examples) could we use to follow up this test. Explain why we should use those tests with justification.Why is it necessary to use a 24-hrold culture in gram staining? What does Gram staining reveal owing to its use in clinical diagnosis? Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. G+ G- 2. Mordant was skipped G+ G- 3. Decolorizing step was omitted G+ G- 4. counterstain was not used? G+ G- 5. Crystal violet and safranin was swapped in position. G+ G- What bacterial specimen represent the following: (a) positive simple staining; and (b) negative simple staining viewed under OIO. Provide details such as specimen and bacterial stain used and reference. Positive Staining 1000 X Negative Staining 1000 XDecolorization in Gram-staining removes safranin from gram-negative cells True False
- Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative stainingIllustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 1. Simple StainingBoth crystal violet and saffron are basic Steve's and maybe used to do simple stains on gram-positive and gram-negative cells this being the case explain how they end up staying in gram-positive and gram-negative cells differently in the Gram stain