he lac operon, determine whether genes will be expressed (on) or silenced (off) given the following: a. Lactose levels are high, glucose is low. b. The lac repressor can no longer bind DNA; lactose and glucose are low.
Q: Predict the level of genetic activity of the lac operon as well as the status of the lac repressor…
A: There are two regulatory factors present in the lac operon of E.coli These are the repressor…
Q: A lac operon containing one mutation was cloned into a plasmid, which was introduced by…
A: the lac operon system present in bacteria
Q: If the above gene is one of the three structural genes of the lac operon that codes for the protein/…
A: The bacterium Ecoli possess a highly effective lactose Metabolism process . The lac operon is a…
Q: Geneticists isolated six trains of E. coli (strains 1-6) whose lac operons had one of the following…
A: LAC operon of E. coli is an example of inducible gene expression system. Lactose acts as inducer…
Q: a) In an experiment, you have changed a DNA sequence directly upstream from a start codon of an…
A: The question says that transcription is not affected but translation is. Therefore, some sequence…
Q: a. The original constitutive operator mutations in thelac operon were all base changes in o1. Why…
A: The original constitutive operator mutations made in the lac operon involved base changes in the O1.…
Q: Six strains of E. coli (mutants 1-6) that had one of the following mutations affecting the lac…
A: The mutations that would prevent the E.coli strain from utilizing lactose include deletion of lacY,…
Q: Strain X-23 has a mutation in the lacI gene resulting in a lacI protein that cannot bind DNA.…
A: The operon model is applicable in bacterial gene regulation and some lower eukaryotes gene…
Q: You have isolated two different mutants (reg1 and reg2) causing constitutive expression of the emu…
A: A constitutive mutation causes the continuous production of the gene product. It means in this…
Q: In the galactose operon of Escherichia coli, a repressor, encoded by the galR gene, binds to an…
A: Galactose operon or gal operon refers to an operon in prokaryotic cells such as Escherichia coli…
Q: On its chromosome, an E. coli cell has a genotype of lacI− lacZ+ lacY+ lacA+. It has an F′ factor…
A: Lac operon is studied extensively by using mutant strains of E.coli. It is studied under the domain…
Q: In the bacterium,Martian coli, it was discovered that the lac operon is positively regulated.…
A: Operon is a functional unit of DNA that consists of cluster of genes under control of promoter.…
Q: A. A mutation is recovered in the gene that encodes the lactose operon repressor protein (LacI).…
A: Lac operon is a simple prokaryotic group of genes for the metabolism of lactose. It consists of…
Q: Let’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively…
A: E.coli is a gram negative bacterium which thrives in the intestine of mammals. E.coli is beneficial…
Q: m. In the figure below, the bacterial activator protein CAP and the Lac repressor have been placed…
A: Introduction :- The lac operon is a group of genes with a single promoter that encode genes for…
Q: If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or…
A: The lac operon or the lactose operon is a mechanism that is involved in the metabolism and transport…
Q: You have isolated a series of mutants affecting regulation of the lac operon. All of these are…
A: The lac (lactose) operon works in the prokaryotic cells and regulates the metabolism of the lactose.…
Q: Glucose absent, lactose absent Glucose absent, lactose present Glucose present, lactose…
A: An operon is found in bacteria and viruses as a genetic regulatory system. The gene coding for…
Q: An E. coli cell acquires a mutation in the gene that encodes CAP. The mutation affects the…
A: The transcription is a process through which information in the DNA molecule is transcribed into…
Q: If CAP is bound to the Promoter of the Lac Operon and the repressor protein is not bound to the…
A: Operon is the prokaryotic gene regulatory system that controls the expression of polycistronic mRNA.…
Q: The lac operon is strongly transcribed when which of the following sugars are present in the growth…
A: The lac operon is an operon or group of genes with a single promoter (transcribed as a single mRNA).
Q: F’ lac I- O+ Z+/ lac I+ Oc Z- the genotype indicated would lead to what type of expression of…
A: Bacteria contain multicistronic arrangements for the expression of genes that are involved in a…
Q: Explain how the data shown indicate that two operator sites are necessary for repression of the lac…
A: Introduction: Lac operon exerts positive control on catabolite repression that leads to an increase…
Q: The lac operon has 4 genes, I, Z, Y and A. For each scenario, tell me the result of the mutation,…
A: The lac operon is an operon or group of genes with a single promoter. The genes in the operon…
Q: What experimental results would indicate that the mutation lacISlacIS is dominant to lacI+lacI+?…
A: The correct option is (e) i.e, In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a…
Q: In lac operon, both gene A and gene B undergo a transcription process. Gene B can only undergo…
A: An operon is a gene regulation mechanism in prokaryotes.
Q: Answer as Directed. Below is the model of a lac operon. lac I lac Z C promoter operator +1 lac Y lac…
A: The prokaryotic gene regulatory system is known as operon system which ultimately responsible for…
Q: For the lac operon, when glucose is absent and lactose is present ___________ None of the…
A: Question - For the lac operon, when glucose is absent and lactose is present ___________…
Q: Let’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively…
A: Escherichia coli contains genes present in the lac operon that are activated in the absence of…
Q: Which of the following environmental conditions would be MOST likely to result in transcription of…
A: The transcription of the lac Oberon in an E.colicell is high levels of lactose and high levels of…
Q: Under what conditions would you expect to see high levels of expression of the structural genes in…
A: Please find your answer in step2:
Q: Consider the trp operon found in E. coli. You have discovered a mutant strain of E.coli that…
A: The genome of the prokaryotic organisms is polycistronic i.e. the genes are controlled by one…
Q: If the above gene is one of the three structural genes of the lac operon that codes for the protein/…
A: Introduction Lactose operon(lac operon) is a system which includes genes that are essential for…
Q: A graduate student studying the pathogenic bacteria Acinetobacter baumannii made cDNA from…
A: Biotechnology is the use of our understanding of biological processes to develop useful applications…
Q: If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or…
A: The lac operon or the lactose operon is a mechanism that is involved in the metabolism and transport…
Q: If glucose levels in the cell are high and lactose is available from the environment, what is the…
A: Bacteria such as E.coli has developed an outstanding system of genes that together regulate the…
Q: For the wildtype E. coli two sugars influence the level of transcription of the lactose operon. Fill…
A: Sugars in the growth media cAMP levels (High or Low) Positive regulation occurs (Yes…
Q: Why is it adaptive for the structural genes for using lactose to be under the control of a single…
A: Each cell can adjust to its surroundings to the best of its abilities. This adaptability is achieved…
Q: Strain ROFL4 has a premature stop mutation in the lacZ gene, resulting in a nonfunctional…
A: Answer: lac Z gene is the gene in lac operon where it is responsible for the formation of…
Q: Many bacterial genes with related functions are arranged in operons, sets of contiguous genes that…
A: Transcription is the first od several steps of DNA based gene expression in which a particular…
Q: You have E. coli growing in HIGH lactose and HIGH glucose. You measure the amounts of protein and…
A: The E.coli is growing in a medium rich in lactose and Glucose both. There are two regulatory…
Q: The fictional bacteria Plumbumbacter aurus converts the lead ions to gold ions and uses the…
A: Gene regulation at the level of transcription in bacteria is achieved by the operon model. Operon…
Q: a)How far along the DNA would the transcription "bubble" formed by RNA polymerase move in 10…
A:
Q: Which of the statements about the attenuator region of the trp operon is TRUE? Choose an answer…
A: It is located upstream of the operator.
Q: For some time, it was not clear whether lac repressor inhibits lac operon transcription by…
A: The binding of the lac repressor to the RNA(ribonucleic acid) polymerase of the lac transcription…
Q: What are the requirements for the lac operon to be actively transcribed? a. Glucose and lactose…
A: Operons are the group of genes which are transcribed under a single promoter. These are found in…
Q: There is a mutation in sequence of the lac operator that prevents the lac repressor from being able…
A: Gene expression is regulated at the level of transcription by operon systems. In prokaryotes,…
Q: The lac operon consisting of lacl, lacZ and lacY genes in addition to all the transcripton factors…
A: Lac operon is an example of an inducible operon here lactose act as a inducer so, in the presence of…
Q: If glucose is not available, but lactose is available from the environment, what is the status of…
A: Points to keep in mind related to Lac operon : 1. Presence of Lactose inhibits repressor protein…
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- If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?In the lac operon (below), how will expression of the genes lacZ and lacy be effect by a mutation in the repressor protein (encoded by lach) that prevents it from binding the operator sequence (lacO) in the absence of lactose? Explain the answer
- The lac operon consists of regulatory regions such as the promoter as well as the structural genes lacZ, lacY, and lacA, which code for proteins involved in lactose metabolism. What would be the outcome of a mutation in lacA?Under what conditions would you expect to see high levels of expression of the structural genes in the lac operon? Group of answer choices Lactose is absent and glucose is absent Lactose is absent and glucose is present Lactose is present and glucose is present None of these Lactose is present and glucose is absentExplain why large amounts of transcript result when a bacterium with a lac Operon is in a medium with no glucose and an abundance of lactose.
- Bacterial DNA containing an operon encoding three enzymes is introduced into chromosomal DNA in yeast (a eukaryote) in such a way that it is properly flanked by a promoter and a transcriptional terminator. The bacterial DNA is transcribed and the RNA correctly processed, but only the protein nearest the promoter is produced. Can you suggest why?There is a mutation in sequence of the lac operator that prevents the lac repressor from being able to recognize and bind to it. What would the status of transcription of the lac operon be under each of the following circumstances? Explain your answers. a-Lactose present, glucose present b-Lactose present, glucose absent c-Lactose absent, glucose present d-Lactose absent, glucose absentIn the lac operon system, do the inducers act at the transcription or translation level? Explain why it acts in that level in a short but concise way.
- Explain how the data shown indicate that two operator sites are necessary for repression of the lac operon. What would the results have been if all three operator sites were required for the binding of lac repressor?What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)In most experiments using the lac operon, researchers use the synthetic inducer IPTG to turn on operon expression, instead of lactose or allolactose. What do you think is the advantage of using IPTG?