Q: starting with four bacterial cells per milliliter in a rich nutrient medium, with a 1-hour lag phase…
A: Bacterial Growth --Bacterial Cell growth is proliferation of bacterium in to daughter cells ,…
Q: Can culture media be sterilized in the hot air sterilizer? Why or why not?
A: Hot air sterilization is a procedure in which the sterilization of the objects are done using hot…
Q: Why does motility media contain a lower amount of agar than ordinary agar?
A: Agar is a phyco-colloid polymer of galactose monomers obtained from red algae (Gelidium and…
Q: Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.
A: Escherichia coli is the gram negative bacteria.
Q: What is the best growth medium that contains glucose that can be used to acclimatize bacteria? and…
A: A growth medium is a liquid, solid, or semi-solid preparation that supports the growth of a…
Q: Is the Tryptoc Soy Agar (TSA) a complex defined medium? Explaining based on its composition? Is TSA…
A: Trypticase soy agar (TSA) and Trypticase soy broth is a growth media for the culturing of bacteria.
Q: What kind of medium might you make to selectively grow a bacterium that lives in your gut?
A: MacConkey agar is used as a selective and differential media for differentiating gut bacteria which…
Q: Given a log phase bacterial culture with 1 x 10^6 cells per ml and a generation time of 30 minutes…
A: Cell multiplication is due to the growth which leads to an increase in the number of bacteria over a…
Q: What is the similarities of differential centrifugation and gradient centrifugation?
A: Similarities between differential centrifugation and gradient centrifugation is process involved…
Q: Why is oil immersion necessary to see bacteria in the laboratory?
A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…
Q: In growing E.coli, why is that (reasons) they do not grow after doubling time under 20 degrees…
A: In a core typical range of its growth temperatures (20 to 37°C), Escherichia coli cells will grow…
Q: In the Messelsohn-Stahl experiment, there should be ______ band(s) in the centrifugation tube after…
A: Meselson and Stahl's experiment is an experiment that supported the semi-conservative replication…
Q: How is the CTSA (Coconut tryptic soy agar) growth medium designed to meet the nutritional need of…
A: Coconut trypic soy agar media are general-purpose, non-selective media that provide enough nutrients…
Q: Can a bacterium be positive for both MR and VP? explain
A: MR(Methyl Red) test is performed to detect glucose fermenting bacteria. The MR test acts as a pH…
Q: how can ultraviolet be used to control microbial growth. which specific UV can be used to control…
A: Ultraviolet (UV) - non-ionizing radiation. - penetration power is less. - It is a physical mutagen.
Q: What is the difference between defined media and complex media?
A: media is a solution in which is rich in nutrients. it is very suitable for the growth of microbes…
Q: What would happen if an ampicillin reagent is added to an Lysogeny broth agar plate that is below 50…
A: Ampicillin is an antibiotic which enables the synthesis of the peptidoglycan of bacterial cells wall…
Q: f my tissue cultures are suffering severely due to alkaloid production and accumulation in the…
A: Cell culture is the technique in which cells isolated from multicellular organisms are grown in…
Q: Please refer to the figure of an agarose gel below to answer the questions. Why is it meant by the…
A: Agarose gel electrophoresis is a fundamental technique in molecular biology that is used to view and…
Q: What is differential medium and differential media? Explain the difference ?
A: Difference between differential medumium - .- A differential medium support the expansion of any…
Q: A growth medium consisting of only known amounts of water, magnesium, calcium chloride, potassium…
A: The growth medium, also called as Nutrient Broth, is a kind of solution which is sterilized to get a…
Q: Which of the following agents if introduced into a growing culture of bacteria would halt growth but…
A: INTRODUCTION Bacteria, also called germs, are microscopic organisms not visible with the naked eye.…
Q: Solid media is usually prepared with a 1-2% final concentration of agar. If these plates were…
A: Solid media is the type of media that is made up of liquid substances like distilled water and other…
Q: What is a pure culture and how can one be obtained
A: Cells are the structural and functional units of life.
Q: On which of the following types of media would you expect untransformed bacteria to grow on?
A: In this question, we have to answer which kind media support the growth of untransformed bacteria.
Q: You are working with an intestinal bacterium of humans, which you have in pure culture (in liquid…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: What makes agar good support for microbial growth?
A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…
Q: Why would cells that are vigorously growing when inoculated into fresh culture medium have a shorter…
A: Growth curves are generally the description of the density of cell populations in liquid culture…
Q: What is the advantage of the plating method over an electronic cell counting method in counting…
A: Bacteria culturing is done in order to grow the bacterial strains that are able to show resistance…
Q: Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium…
A: Bacteria are organisms that are found almost everywhere on earth and are free-living cells that are…
Q: Can culture media be sterilized in the hot air oven? why or why not?
A: Sterilization is the process of deactivating, killing, or removing any kind of microorganism (or…
Q: What is a complex medium? and What are the basic components of a culture medium?
A: Medium is defined as the solid or liquid substratum on which the cells or organ can grow for further…
Q: Which of the following samples are expected to cause a decrease in absorbance over time when…
A: Ensuring adequate light reaching a detector by using water colloidal solutions in light or X-ray…
Q: Can simply observing the colonies formed on agar plates and the results of a few biochemical tests…
A: Bacteria are of many types. They can be classified based on different properties such as cell wall…
Q: Why did we inoculate the KIA slant by stabbing it with an inoculating needle rather than spread the…
A: Inoculating needles are used to inoculate microorganism like - Bacteria from one solid culture media…
Q: Why is it necessary to store the molten agar in a water bath at 50 degrees Celsius?
A: Introduction:- Agar is made up of two polysaccharides: agarose and agaropectin, with agarose…
Q: If my cultures are suffering severely due to alkaloid production and accumulation in the medium. In…
A: Alkaloids are basically the natural substances that can be used largely for human medication.…
Q: You have a starter culture containing 8 x 109 cells per mL, from which you take 10 mL to inoculate a…
A: The generation time of culture is considered to be the time at which it doubles and the mean growth…
Q: 1. You were given a mixed nutrient agar broth culture of bacteria. a. How will you determine the…
A: Nutrient agar medium is used to cultivate microbes supporting the growth of a wide range of…
Q: What chemical ingredients make MSA differential? How (in your own words) do those chemical…
A: Introduction A liquid medium known as enrichment media contains specific selective agents,…
Q: Which dye will not kill microorganisms if a wet preparation is made? Why?
A: Microorganisms are defined as organisms that cannot be viewed by the unaided eyes and are studied…
Q: ar contains salt to prevent the growth of most microbes and mannitol plus a pH indicator to detect…
A: In order to produce and culture different types of microbial species, a culture media is required.…
Q: Why are plated media stored in an inverted position?
A: Plated media enables the isolation of microorganisms from samples or specimens.
Q: Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind…
A: The selective ingredients of MSA the bile salts and the dye, crystal violet which inhibit the growth…
Q: Why is bacterial culture useful to grow bacteria?
A:
Q: Why should agar media be completely dissolved before they are dispensed in tubes and plates? What…
A: Introduction A growth medium, also known as a culture medium, is a solid, liquid, or semi-solid…
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- In preparing bacterial smear, why do we need to pass the slide over the flame? Enumerate the advantages derived from staining bacteria. In Gram staining method, list the reagents and state their role for each step of the procedure? Conclusion?If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperationWhy do we use gel electrophoresis?
- Why is it important that the agarose is exposed to ethidium bromide, which is either added to the gel itself or mixed to the staining solution? Explain the principle behind its use.You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is the dilution factor?How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium?
- What is diluent? Why is it useful for working with bacterial samples?Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?