Which of the following samples are expected to cause a decrease in absorbance over time when performing light scattering experiments with bacteria? HEW, CARB 1 and Lysozyme standards Lysozyme standards and CARB 1 Lysozyme standards HEW, LOAD, and CARB1 You take 0.5 mL of Carb 1 and add it to 2.0 mL of buffer. You then add 7.5 mL Biuret to this sample. The total dilution of Carb 1 in the final sample is 9 20 10 0.05
Q: Consider a protein with a beta conformation sequence EAGQVHRGP a) Which residue(s) can be substitute...
A: Beta conformation in protein: Beta sheet, beta-turn etc are beta conformation in protein. Beta sheet...
Q: Briefly describe the mechanism of action of lipid soluble chemical messengers. Include their transpo...
A: Signal molecules are chemical compounds. The signal molecules are synthesized by signaling cells and...
Q: Giveee a sufficient biosynthesis for this compound starting from acetyl CoA, S-alanine, S- adenosyl ...
A: Here compound 14 is synthesized from L-Phenylalanine, SAM, L-alanine, and Acetyl-CoA in multistep p...
Q: Vitamin A in vision Vitamin C in collagen formation Vitamin K in blood clothing
A: Vitamins are the organic compounds that requires in small quantities. Vitamins have wide variety of ...
Q: What factors affect blood glucose levels? Explain
A: Several factors affect glucose levels in the blood, like food, physical activity, medicines are the ...
Q: Protein solubility in aqueous solutions is independent of ionic strength of the solution. True or...
A: Proteins are biomolecules composed of amino acids. Amino acids have ionizable groups, which can acce...
Q: Compare and contrast the formation of mRNA in bacterial and eukaryotic cells. How do the differences...
A: Gene expression is the process by which genes are turned on in a cell to form RNA and proteins. This...
Q: The restriction digests of the cloned Drosophila gene can provide direct visible evidence of a mutat...
A: We can use the technique of Southern blotting to detect and isolate the DNA fragment with the Gene O...
Q: 3. In Drosophila, an X-linked recessive mutation, scalloped (sd) causes irregular wing margins. Diag...
A: A) a scalloped female is crossed with a normal male: - Xsd Xsd × X Y Xsd Xsd X Xsd X Xsd X ...
Q: unfold the protein
A: here they are talking about protein unfolding. The tension applied by the cantilever during protein ...
Q: Analysis of a octapeptide revealed the presence of the following products: 2 Arg, 1 Gly, 1 Met, 1 Tr...
A: Edman Degradation – This is method of peptide sequencing. In this method amino terminal residue is l...
Q: QUESTION 10 n polynucleotides, the phosphodiester bond is between the O 3' OH from the 3' end, and 4...
A:
Q: What are nucleosides?
A: Nucleic acid are the largest biomolecules which are found in genetic information. There are two comm...
Q: Which of the following statements are TRUE about eukaryotes? (i) They are cells with a nucleus. (ii)...
A: Cell is the structural and functional unit of life. It can be classified into prokaryotic and eukary...
Q: give one example of charbohydrate being used as information molecule, explain
A: A carbohydrate is a biomolecule made up of carbon (C), hydrogen (H), and oxygen (O) atoms, generall...
Q: Cecilia is a l45-pound woman who consumes five standard drinks (14 gr of alcohol each). Estimate how...
A: Widmark Formula: This is a formula suggested by Swedish Scientist and forensic toxicologist Erik Wid...
Q: Diagram and describe Van Der Waals interactions between valine and phenylalanine that help to stabil...
A: Proteins structure has different levels of conformation, which includes the primary structure, secon...
Q: Compare and contrast the structure of lipids, nucleic acids, carbohydrates, and proteins (with refer...
A: Carbohydrates, proteins, lipids and nucleic acids are biomolecules that are important for the physio...
Q: Interpret completely the following Hepatitis profile. Explain your answers. Acute Hepatitis B; Immun...
A: Hepatitis B is a liver disease caused by hepatitis B virus or HBV. This can be preventable by vaccin...
Q: Our growing understanding of how proteins fold allows researchers to make predictions about protein ...
A: The proteins are composed of twenty standard amino acids that differ from each other on the basis of...
Q: The interior core of globular proteins is typically composed of non-polar amino acid residues becaus...
A: Proteins are made up of polypeptide chains of amino acids. The amino acids arrange themselves to for...
Q: Below are the structures of three phosphate-containing molecules. Which one has the lowest Phosphory...
A: Phosphate group is containing one phosphate group atom attached to four oxygen molecule.Various mole...
Q: Is Sodium Phosphate A Competitive Or Noncompetitive Inhibitor Of Alkaline Phosphatase?
A: Enzyme is a biocatalyst. It speeds up biochemical reactions. It is mostly protein based though some ...
Q: 8 10 4 1 O SSDNA O All Polynucleotides QUESTION 6 The carbon labeled in the structure below is the 3...
A: The given structure indicates an ATP nucleotide. Nucleotides are the basic structural units of a nuc...
Q: Do we need to denature proteins? Why?
A: Heating, pH fluctuations, high concentrations of chemicals like urea, or reducing agents that break ...
Q: Using a semi-permeable membrane, dialysis allows the removal of salt ions prior to chromatography. T...
A: Dialysis is a separation process that uses selective and passive diffusion via a semi-permeable memb...
Q: - TRUE about the peptide bond:
A: With the loss of a water molecule, a covalent link is formed between the -amino group of one amino a...
Q: Which has better emulsifying properties, soap or detergent. Explain your answer using the concept of...
A: Emulsifiers are those substances that decrease the surface tension between two phases which are comp...
Q: Which of the following factors favors the formation of the Random DNA Coil? a Enthalpy b Bas...
A: Some parts of the protein chain do not form regular secondary structure or have a consistent hydroge...
Q: A HEPTAPEPTIDE that punctures the bacterial cell wall has just been recently isolated from the venom...
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids con...
Q: A polypeptide with a net positive charge at physiologic pH (~7.4) most likely contains amino acids w...
A: are non-polar. Doesn't contribute to charge o the polypeptide.
Q: Assessing a remedy for flatulence Certain foods, particularly beans and legumes, contain substance...
A: Carbohydrates, often known as saccharides, are sugar molecules. Carbohydrates are one of three prima...
Q: In the acid- catalyzed hydrolysis of starch, how can you tell when the hydrolysis of starch is compl...
A: Starch is a branched polysaccharide that is made up of glucose units linked by Alpha 1,4 ans 1,6 Gly...
Q: What does a graph of fluorescent relative units (RFUs) vs. time (s) tell about kinetic parameters fo...
A: Fluorescent relative unit (RFU) : It is a unit of measurement employed for DNA Analysis in electrop...
Q: 32. Which one of the following is NOT a typical characteristic of the active site of an enzyme? A) T...
A: Substrate molecules bind to the enzyme, which then undergoes a chemical reaction. The active site is...
Q: • Draw the 2 anomeric ring structures for D-glucose, using CHO the information in the straight- н-с ...
A: Glucose is a monosaccharide. It is aldohexose. It has six carbon atoms and a functional group presen...
Q: Chargaff rule applies to a ss RNA b All Polynucleotides c ssDNA d dsDNA
A: Chargaff rule was stated by Austrian born chemist Erwin Chargaff. The specifies about the 1:1 ratio ...
Q: Which of the following is a drawback of using base hydrolysis during amino acid composition? O Proto...
A: A protein is consisted of several amino acids bond together in sequence with peptide bond. To analyz...
Q: Study the given structures below. Which of the following are enantiomers?" -OH но- H- HO- но- HO- но...
A: Enantiomers are the molecules that are exactly mirror images of each other. They cannot be superimpo...
Q: 55 Mutations in the gene encoding the glycolytic enzyme triose phosphate isomerase lead to severe me...
A: Introduction: Triose phosphate isomerase deficiency is a severe disorder that is characterized by s...
Q: An infant with corneal clouding has dermatan sulfate and heparan sulfate in his urine. Decreased act...
A: Introduction: The syndrome is also known as mucopolysaccharide type I (MPS I). It is an autosomal r...
Q: Draw the structure of the wax composed of stearic acid and a straight-chained alcohol with 30 carbon...
A: Waxes are simple lipids composed of fatty acids (saturated and unsaturated) which are esterified to ...
Q: 1. Protein that will most strongly bind to an anion exchange column: [ Select] 2. Protein that will ...
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids conta...
Q: A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown b...
A: The rate of enzyme catalyzed reactions is directly proportional to the substrate concentration. But,...
Q: The cell concept CANNOT apply to ____
A: Cell theory (a unifying principle of biology) was formulated by Schleiden and Schwann. Cell theory s...
Q: What is experiment of Thermodynamics of Small Oligomeric Duplex DNA Denaturation.
A: Double-stranded DNA is the most common kind of DNA molecule found in nature. The strands can be unwo...
Q: Enzymes increase the rate of a chemical reaction by providing an alternative path with a lower activ...
A: TRUE
Q: Q24. Choose the best answer for the following questions.
A: Amino acids are building blocks of proteins and also important as a precursor for the biosynthesis o...
Q: a reasonable biosynthesis for Compound 14 starting from Propose alanine, S-phenylalanine and ATP.
A: Given compound in a figure is Aspartame. It is an artificial sweetener and is made by dipeptide of a...
Q: 2. How does SDS-PAGE separate proteins and peptides from each other? Explain
A: 2. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is an immunological techniqu...
Which of the following samples are expected to cause a decrease in absorbance over time when performing light scattering experiments with bacteria?
HEW, CARB 1 and Lysozyme standards |
||
Lysozyme standards and CARB 1 |
||
Lysozyme standards |
||
HEW, LOAD, and CARB1 |
-
You take 0.5 mL of Carb 1 and add it to 2.0 mL of buffer. You then add 7.5 mL Biuret to this sample. The total dilution of Carb 1 in the final sample is
9
20
10
0.05
Trending now
This is a popular solution!
Step by step
Solved in 4 steps
- Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?Which of the following sample(s) is NOT expected to cause a SUBSTANTIAL decrease in absorbance over time when performing light scattering experiments with bacteria? [Choose the best possible answer] LOAD and Bacteria alone Lysozyme standards HEW and CARB1 Bacteria aloneAssume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If we can order 5 milligrams of fibronectin for $871.00, how much would it cost to have enough to use every well of the 24-well culture plate?
- A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture into a 9 ml LB medium. After this, from 10-4 and 10-5 dilution tubes 100µl were plated onto LB agar plates. Upon overnight incubation at 37°C, 200 colonies were counted in 10-4 and 22 colonies were present on 10-5 plates. How many colony-forming units were present per ml of the original culture? If the formula for CFU/ml =no. Of colonies/dilution factor*volume of culture plateHow would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume? suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10^-1 dilution of the entire culture. Explain how you would do this. Show your calculations.You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is the dilution factor?
- In your opinion, for efficient multiphoton excited fluorescence microscopy, would it be better to use a 100 fs 80 MHz laser pulse train or a 200 fs 80 MHz laser pulse train for imaging biological samples? Please explain your answer using equations 1 and 2 <I(t)2> = gP <I(t)>2 / (Rτ) ..............equa (1) τout = τin (1 + 7.68(D/τ2in)2) 1⁄2 ................equa (2) D is the total dispersion in femtoseconds squared gp is a unitless factor that depends on the temporal laser pulse shape (0.66 for a Gaussian pulse shape), τ is the full-width half-maximum (FWHM) of time average valueThe acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in performing the acid-fast stain technique because of its thin cell wall. However, if this organism is used for Gram-stain, it can also take the Gram stain reaction. Why is this so?What are the things you need to consider when doing the following: a. Preparing/determining the concentration of the agarose gel- b. Loading the samples- c. Choosing the stain- d. Placing the electrodes on the power source- Briefly describe the function/s of each of the following: e. Gel f. Comb g. Stain h. Running buffer j. Molecular weight ladder/DNA size standard k. Micropipette l. Tracking dye m. Anode (red) n. Cathode (black) o. Microwave oven
- how many ml of sterile water injection must be mixed with two 4 ml vials of sterile hypertonic NaCl 7% USP to make the mixture isotonic? pharIn a mixture of five proteins listed, draw an elution profile (Absorbance vs. mL eluted, arbitrary) for the purification of the listed proteins on a gel filtration chromatography resin: cytochrome c (pI = 5.4; Mr = 13,000), immunoglobulin G (pI = 7.3; Mr = 145,000), ribonuclease A (pI = 9.6; Mr = 13,700), RNA polymerase (pI = 6.3; Mr = 450,000), human serum albumin (pI = 5.4; Mr = 68,500). Label your elution peaks. Draw a sketch of an SDS-PAGE, reflecting the mobility of the above mixture as they elute from the column. Label you protein bands.You dilute 1 mL of bacterial culture with 99 mL of water. What is the resulting dilution factor?