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- Why does motility media contain a lower amount of agar than ordinary agar?What is the EFFECT and RATIONAL of Molten agar mixed with bacterial suspension has temperature higher than 45 oC.Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?
- What type of agar is MacConkey agar?The following ingredient list represents which type of growth media? Beef extract 1.5 g Yeast extract 3.0 g Peptone 6.0 g Glucose 1.0 g Agar 15.0 g water 1000 ml pH 6.6In a nutrient broth, where do microorganisms acquire their requirements for mineral elements like Ca, Fe, etc. from? Can gelatin be used in culture media preparation? How is it different from agar? During sterilization, pH of culture media may change. How can this be fixed? How does pH affect the shelf life of unsterilized media?
- Aside from Paraffin, what is your recommended infiltrating medium for routine purposes? why?What is the advantage of the plating method over an electronic cell counting method in counting cells? Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?When is sterilization by filtration preferred over sterilization by heat? Why? What makes heat an effective sterilizing agent? Why are the conditions required for sterilization greater for dry heat (160-180°C for 1-2hrs) than for steam (121°C for 15min)?
- During media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen storage in crypto preservation? In addition, why are antibiotics not used in the cell freezing media? Why FBS concentration higher than 10% (10% is the standard concentration in complete growth media) is recommended in cell freezing media?What concentration of ethanol is considered optimum for disinfectant? Why not 100%?