Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
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A: Nitrifying bacteria are a small group of aerobic bacteria belonging to the family Nitrobacteraceae…
Q: Phenyl Ethyl Alcohol agar encourages the growth of what type of microorganisms?
A: Answer - Option A - Gram positive bacteria
Q: Why del factor is important in media sterilization?
A: Sterilization, which was called by various means such as Del factor, Nabla factor and the…
Q: Why is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet…
A: Introduction : Sabouraud agar, often known as Sabouraud dextrose agar (SDA), is a peptone-containing…
Q: Agar is a solidifying agent used in media preparation. What is its origin?
A: Agar gel is a polysaccharide which can become liquefied at temperatures ranges from 85°C to 90°C and…
Q: In the laboratory, autoclaving is required to prepare media for the growth of What type of method of…
A: The most effective method by which we sterilize the laboratory instruments is known as autoclaving.
Q: What concentration of agar is used in a semisolid medium for motility determination?
A: Motility is a feature characteristic of only some bacteria. This ability allows the bacteria to move…
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A: High yield of yeast cannot be attained unless air in a huge quantity is injected in fermentation…
Q: Why are semi-solid media such as TTC deeps suitable to test motility of bacteria?
A: There are several parameters which need to be studied in microbiology about different…
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A: Clostridium tetani is a gram-positive sporulating bacterium. It is found in soil and forms…
Q: How is the CTSA (Coconut tryptic soy agar) growth medium designed to meet the nutritional need of…
A: Coconut trypic soy agar media are general-purpose, non-selective media that provide enough nutrients…
Q: Name the source from where agar-agar is obtained.
A: Agar-agar is a gelatinous substance that is formed by a combination of linear polysaccharide agarose…
Q: A broth culture is initially inoculated with a single bacterial cell. For the first hour the…
A: * Given that The bacteria has generation time of 30 minutes. So it takes about thirty minutes to…
Q: Why is the batch bioreactor is suitable in milk fermentation?
A: Batch bioreactor is a closed system and the process lasts until the nutrients are consumed.
Q: why macconkey agar and mannitol salt are regard as salective and deffrental medium?
A: A culture medium is just water and nutrients that support microbic growth. Primary culture media for…
Q: What advantages do solid media offer for the isolation of microorganisms?
A: “Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: why do We need to Wait for the iron Agar to set and then Add another thin layer of 45°C Iron Agar?
A: The test that uses iron agar is the Triple Sugar Iron (TSI) Test and is used to determine the…
Q: why is it important that a small amount of inoculum be used in preparation of an agar slant
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Q: You have a broth medium that you would like to turn into a solid medium in order to do streak…
A: The "nutrient broth" is essentially nutrient agar without the solidifying agent. They stay liquid at…
Q: hy is nutrient broth considered as a universal growth medium for bacteria?
A: Nutrient Broth is a general-purpose medium for growing a wide range of fastidious and non-fastidious…
Q: What would you expect to see if you cultured Gram-positive, lactose-fermenting bacteria on MacConkey…
A: Given: What would happen if you culture gram positive, lactose fermenting bacteria in MacConkey…
Q: In a nutrient broth, where do microorganisms acquire their requirements for mineral elements like…
A: Microorganisms are grown in laboratories using culture media. Culture media contains all the…
Q: Why is agar ideal for microbial cultivation?
A: The microbial cultivation is the process in which microorganisms are cultivated in the laboratory,…
Q: On which of the following types of media would you expect untransformed bacteria to grow on?
A: In this question, we have to answer which kind media support the growth of untransformed bacteria.
Q: Which of these bacterial characteristics could be evaluated with one of the rapid identification…
A: Arabinose Fermentation Test is used to determine the if the microbes can ferment the carbohydrate…
Q: Which of the following best descrives UTI agar? Selective growth media; Differential Growth Media;…
A: A growth or culture medium is a liquid, solid or semi-solid medium, which is designed in a way that…
Q: Why does the oxidase test must be performed from blood agar or another medium without a fermentable…
A: Biochemical tests are used to identify microbial diversity based on variations in the biochemical…
Q: What makes agar good support for microbial growth?
A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…
Q: WHAT IS THE TYPE OF Growth patterns on Agar Slant and Broth Medium
A: Growth and morphogenesis of plant is determined by the composition of culture media. There are few…
Q: What is the name of this media? What type of media is it? What does the yellow color indicate? What…
A: Selective media generally selects for the growth of a desired organism, stopping the growth of or…
Q: What is the purpose of adding sodium bicarbonate to a culture medium?
A: >> The purpose of adding sodium bicarbonate to animal culture medium is that it acts as a…
Q: why are psychrotrophic bacteria incubated at 7 degrees
A: Each species of microbe has a preferred temperature that lies somewhere between its characteristic…
Q: Why does dry heat require higher temperatures for longer time periods to sterilize than moist heat
A: Sterilization may be defined as the process which help in the complete removal of all…
Q: Why is a GasPak anaerobic jar necessary for the culture of anaerobes on Brewer’s anaerobic agar…
A: Brewer’s anaerobic agar and fluid thioglycollate medium (FTM) are used to grow bacteria. Both of…
Q: Why does saliva not serve as a good microbialgrowth medium?
A: Saliva is the watery, frothy substance that is produced in the mouths of some animals, including…
Q: What is the main difference between plates before and after centrifugation? What may be the purpose…
A: Answer: CENTRIFUGATION = It is the process involving mechanical force that is centrifugal force,…
Q: Why do you need to use antifoams in fermentation medium? How you could avoid from contamination…
A: Fermentation is a biochemical process that involves chemical changes in organic substrates, such as…
Q: What are the culture media that can be used for cultivating non-specific and specific…
A: A microbiological culture medium is a substance that promotes the growth, support, and survival of…
Q: What types of bacteria grow on nutrient agar?
A: Nutrient agar is a nutrient-rich medium that allows the growth of a wide range of organisms in the…
Q: If my cultures are suffering severely due to alkaloid production and accumulation in the medium. In…
A: Alkaloids are basically the natural substances that can be used largely for human medication.…
Q: What is the proper term for an organism whose optimum growth range is between 20 and 50C but can…
A: The temperature range in which the microbe grows corresponds to the temperature at which its enzymes…
Q: What is diluent? Why is it useful for working with bacterial samples?
A: Dilution is done to decrease the concentration of the original sample that is being tested. Dilution…
Q: How many different types of colonies can you find on the blood agar plate? describe each. (As shown…
A: Blood agar is general medium that is provided with the nutrients and can be use as a general growth…
Q: Why are plated media stored in an inverted position?
A: Plated media enables the isolation of microorganisms from samples or specimens.
Q: Why did we add agar after we measure the pH?
A: pH means p stands for potential or power.H stands for Hydrogen atom.It is the power of the Hydrogen…
Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: Which is more Suitable for long-term storage: agar slant or plate? Explain why?
A: In order to isolate the bacteria from samples, they need to be inoculated into a growth medium which…
Q: Why should agar media be completely dissolved before they are dispensed in tubes and plates? What…
A: Introduction A growth medium, also known as a culture medium, is a solid, liquid, or semi-solid…
Why does motility media contain a lower amount of agar than ordinary agar?
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- What concentration of agar is used in a semisolid medium for motility determination? How does that compare to a typical solid medium ? Explain.What type of agar is MacConkey agar?During media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?
- Agar is a solidifying agent used in media preparation. How and why does the agar concentration in semisolid media differ from conventional solid media?What is the advantage of the plating method over an electronic cell counting method in counting cells? Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?Agar is a solidifying agent used in media preparation. What is its origin?