How can you calculate the concentration of a solution with UV-vis spectroscopy if you do NOT know the molar absorption coefficient? (a) Add blue dye to the sample and then subtract the blank sample's absorbance. (b) Use Beer's Law by assigning the molar absorption coefficient a value of 1 (e = 1). (c) Create a calibration curve which is a plot of absorbance vs concentration. (d) Calculate the rate constant. (e) Divide the measured absorbance by the product of l´e.

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter26: Molecular Absorption Spectrometry
Section: Chapter Questions
Problem 26.1QAP
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Question 1.4
How can you calculate the concentration of a solution with UV-vis
spectroscopy if you do NOT know the molar absorption
coefficient?
(a) Add blue dye to the sample and then subtract the blank
sample's absorbance.
(b) Use Beer's Law by assigning the molar absorption
coefficient a value of 1 (e = 1).
(c) Create a calibration curve which is a plot of absorbance vs
concentration.
(d) Calculate the rate constant.
(e) Divide the measured absorbance by the product of I´e.
Transcribed Image Text:Question 1.4 How can you calculate the concentration of a solution with UV-vis spectroscopy if you do NOT know the molar absorption coefficient? (a) Add blue dye to the sample and then subtract the blank sample's absorbance. (b) Use Beer's Law by assigning the molar absorption coefficient a value of 1 (e = 1). (c) Create a calibration curve which is a plot of absorbance vs concentration. (d) Calculate the rate constant. (e) Divide the measured absorbance by the product of I´e.
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