Malin just discovered a new protein, Malinase and for it not to unfold and loseits function, he realized that the protein needs to be constantly immersed in a pH = 11.90solution. He is therefore interested in preparing a 50.0 mL 0.080 M pH = 11.90 buffer tocontain the protein.He listed down the acid solutions available to his disposal, and also looked for their aciddissociation constant (Ka) values.ACID2.00 M NITROUS ACID (HNO:)2.00 M ACETIC ACID (CH,COOH)2.00 M ASCORBIC ACID (H:Asc)Ka:4.00 x 10N/A1.76 x 105N/A7.90 x 10 1.60 x 102KazКазN/AN/AN/A2.00 M PHOSPHORIC ACID (H:PO4) 7.52 x 10 6.23 x 10 4.28 x 103A.Identify which weak acid system is best to use in preparing the buffer? Why?В.Identify the species which will make up the buffer system?C.prepare the buffer. Calculate the amount (in mmoles) of each of the components necessary toD.Indicate how Malin is supposed to prepare the buffer from 2.00 M of theacid and/or 4.00 M N2OH.E.Calculate the resulting pH if 5 drops (1 ml = 20 drops) 0.500 M HCI wasaccidentally added to the buffer.

Given the required pH of the buffer solution = 11.90 Volume of the buffer required = 50.0 mL Total…

Malin just discovered a new protein, Malinase and for it not to unfold and lose its function, he realized that the protein needs to be constantly immersed in a pH = 11.90 solution. He is therefore interested in preparing a 50.0 mL 0.080 M pH = 11.90 buffer to contain the protein. He listed down the acid solutions available to his disposal, and also looked for their acid dissociation constant (Ka) values. ACID Ка Каз Кa N/A N/A 4.00 x 10* 2.00 M NITROUS ACID (HNO:) 2.00 M ACETIC ACID (CH,COOH) 2.00 M ASCORBIC ACID (H;Asc) 2.00 M PHOSPHORIC ACID (H;POA) 7.52 x 10 6.23 x 10 4.28 x 103 N/A 1.76 x 10 N/A 7.90 x 10 1.60 x 102 N/A A. Identify which weak acid system is best to use in preparing the buffer? Why? В. Identify the species which will make up the buffer system? C. prepare the buffer Calculate the amount (in mmoles) of each of the components necessary to D. Indicate how Malin is supposed to prepare the buffer from 2.00 M of the acid and/or 4.00 M N2OH. E. Calculate the resulting pH if 5 drops (1 ml = 20 drops) 0.500 M HCI was accidentally added to the buffer.

Malin just discovered a new protein, Malinase and for it not to unfold and lose its function, he realized that the protein needs to be constantly immersed in a pH = 11.90 solution. He is therefore interested in preparing a 50.0 mL 0.080 M pH = 11.90 buffer to contain the protein. He listed down the acid solutions available to his disposal, and also looked for their acid dissociation constant (Ka) values. ACID Ка Каз Кa N/A N/A 4.00 x 10* 2.00 M NITROUS ACID (HNO:) 2.00 M ACETIC ACID (CH,COOH) 2.00 M ASCORBIC ACID (H;Asc) 2.00 M PHOSPHORIC ACID (H;POA) 7.52 x 10 6.23 x 10 4.28 x 103 N/A 1.76 x 10 N/A 7.90 x 10 1.60 x 102 N/A A. Identify which weak acid system is best to use in preparing the buffer? Why? В. Identify the species which will make up the buffer system? C. prepare the buffer Calculate the amount (in mmoles) of each of the components necessary to D. Indicate how Malin is supposed to prepare the buffer from 2.00 M of the acid and/or 4.00 M N2OH. E. Calculate the resulting pH if 5 drops (1 ml = 20 drops) 0.500 M HCI was accidentally added to the buffer.

Organic Chemistry

9th Edition

ISBN:9781305080485

Author:John E. McMurry

Publisher:John E. McMurry

Chapter26: Biomolecules: Amino Acids, Peptides, And Proteins

Section26.SE: Something Extra

Problem 50AP: The -helical parts of myoglobin and other proteins stop whenever a proline residue is encountered in...

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A typical amino acid with one amino group and one carboxylic acid group, such as serine can exist in water in several iconic forms. Suggest the forms of the amino acid at low pH and at a high pH. Amino acids generally have two pKa values, one in the range of 2 to 3 and the other in the range of 9 to 10. Serene, for example, has pKa values of 2.91 and 9.21. Using species such as acetic acid and ammonia as models, suggest the origin of the two pKa values. Glutamic acid is an amino acid that has three pKa’s: 2.10, 4.07, and 9.47. Draw the structure of glutamic acid and assign each pKa to the appropriate part of the molecule. An unknown amino acid is titrated with strong base, producing the following titration curve. Which amino acid are likely candidates for the unknown?
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