How is the identity of the different Drosophila imaginal discs established in the embryo—for example, how is it determined whether a disc will form a wing, a leg, or some other structure? b.) Dominant mutants in the gene Antennapedia result in fore legs forming in the location where antennae are expected. How does this mutant phenotype illustrate your response to Part a

How is the identity of the different Drosophila imaginal discs established in the embryo—for example, how is it determined whether a disc will form a wing, a leg, or some other structure? b.) Dominant mutants in the gene Antennapedia result in fore legs forming in the location where antennae are expected. How does this mutant phenotype illustrate your response to Part a

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 16TYK

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In recent years, techniques have been developed to clone mammals through a process called nuclear transfer, in which the nucleus of a somatic cell is transferred to an egg cell from which the nuclear material has been removed . Research has demonstrated that when a nucleus from a differentiated somatic cell is transferred to an eggcell, only a small percentage of the resulting embryos complete development, and many of those that do die shortly after birth. In contrast, when a nucleus from an undifferentiated embryonic stem cell is transferred to an egg cell, the percentage of embryos that complete development is significantly higher (W. M. Rideout, K. Eggan, and R. Jaenisch. 2001. Science 293:1095–1098). Propose a possible reason for why a higher percentage of cloned embryos develop successfully when the nucleus transferred comes from an undifferentiated embryonic stem cell.
When doing a lab that involves Extraction of Genomic DNA from adult Drosophila melanogaster. - What are the controls you should use and why use these? What is special about the genomic DNA? What do you expect to find (using these genomic DNA samples)? please help!
Another way to study the role of proteins (e.g., transcription factors) that function in development is to microinject the mRNA that encodes a protein, or the purified protein itself, into an oocyte or embryo, and then determine how this affects the subsequent development of the embryo, larva, and adult. For example, if Bicoid protein is injected into the posterior region of an oocyte, the resulting embryo will develop into a larva that has anterior structures at both ends. Based on your understanding of the function of each developmental gene, what would be the predicted phenotype if the following proteins or mRNAs were injected into normal oocytes? A. Nanos mRNA injected into the anterior end of an oocyte B. Antp protein injected into the posterior end of an embryo C. Toll mRNA injected into the dorsal side of an early embryo
When doing a lab that involves Extraction of Genomic DNA from adult Drosophila melanogaster. - What are the controls you should use and why use these?
Most organisms display a circadian rhythm, a cycling of biological processes that is roughly synchronized with day length. In Drosophila, pupae eclose (emerge as adults after metamorphosis) at dawn. a)Using this knowledge how would screen for Drosophila mutants that have an impaired circadian rhythm? b)In each case, how would you clone the genes you identified by mutation?
Give typing answer with explanation and conclusion What characteristics of the DSX protein enable the female- and male-specific isoforms of DSX to regulate the same genes but with different outcomes in female and male development? [multiple answers possible] A.The two isoforms are different alleles of the same gene B.The two isoforms have different DNA-binding domains C.The two isoforms are lncRNAs involved in dosage compensation D.The two isoforms differ in their activation domain E.The two isoforms share the same activation domain F. The two isoforms share the same DNA-binding domain
2. a. You want to create a genetic construct that will express GFP in Drosophila. In addition to the GFPcoding sequence, what DNA element(s) must youinclude in order to express this protein in flies if theconstruct were integrated into the Drosophila genome? Where should such DNA element(s) be located? How would you ensure that GFP is expressedonly in certain tissues of the fly, such as the wing?b. Suppose you insert the GFP coding region plus allof the DNA elements required by the answer to part(a)—except the enhancer—between inverted repeatsfound at the ends of a particular transposable element.Because all of the DNA sequences located betweenthese inverted repeats can move from place to placein the Drosophila genome, you can generate manydifferent fly strains, each with the construct integrated at a different genomic location. You now examine animals from each strain for GFPfluorescence. Animals from different strains showdifferent patterns: some glow green in the eyes,others in…
For the Igf2 gene, where do de novo methylation and maintenancemethylation occur?a. De novo methylation occurs in sperm, and maintenancemethylation occurs in egg cells.b. De novo methylation occurs in egg cells, and maintenancemethylation occurs in sperm cells.c. De novo methylation occurs in sperm, and maintenancemethylation occurs in somatic cells of offspring.d. De novo methylation occurs in egg cells, and maintenancemethylation occurs in somatic cells of offspring.
Polarity of oocyte of DrosophilaWhat are the cells surrounding the oocyte? Are they germ-line or somatic in origin?
Discuss Concepts The genome of the yeast Saccharomyces cerevisiae is only about 0.4% the size of the human genome, yet itcontains about 30% as many genes as are in the human genome. Given that, which of the features of the human genome would you expect to find many fewer of in the yeast genome?
Compare and contrast the experimental advantages and disadvantages of Drosophila, C. elegans, mammals, and Arabidopsis.
In somatic cell nuclear transfer, the nucleus of a somatic cell is inserted into an enucleated egg. The resulting embryo is implanted into a surrogate mother. The nuclear genome of the resulting cloned animal will genetically identical to that of ["the original somatic cell", "the surrogate mother", "the enucleated egg"] , and the mitochondrial DNA of the cloned animal will be identical to that of ["the somatic cell", "the enucleated cell", "the surrogate mother"] . Pick answers within quotation marks to fill in the blanks.