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How will 100mM Tris-Cl buffer be prepared?
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- 50 mL of a sample known to contain Mg+2 was taken and titrated with 0.075 M EDTA standard solution by adding pH 10 buffer and EBT indicator. Since the EDTA consumption is 10.5 mL, what is the amount of magnesium in the sample in ppm?Calculate the amount of solid in grams you would need to measure to prepare 50.0 mL of 0.100M sodium acetate buffer (MW 136.08).What is the basis of the ‘40000’ in the equation? Note: this is about "DETERMINATION OF WATER HARDNESS BY EDTA TITRATION"
- hat weight of sodium formate must be added to 400 ml of 1M formic acid to produce a buffer solution that has a pH of 3.5 (pKa= 3.75, MW=68)What is the potential at equivalence point in the titration of 20 mL of 0.050 M U4+ with 0.100 M Ce4+? Assume both solutions contain 1.0 M sulfuric acid.In order to adjust the EDTA solution, 10 mL of 0.01M Ca + 2 sample was taken and 10 mL of pH 10 buffer was added on it and it was completed to 100 mL. Then, two drops of EBT indicator were added and titrated with EDTA. Since the consumption is 13 mL, what is the concentration of EDTA solution in terms of molarity?
- List four methods of detecting the end-point of an EDTA titration and explain each.Tris or tris(hydroxymethyl)aminomethane is an organic buffering reagent most often used in nucleic acid extractions. Tris (MW = 121.14 g/mol) has a pKa of 8.07 at 25oC, with a white crystalline powder appearance. What is the buffering range/s of Tris buffer? How would you prepare 500mL of a 0.200 M Tris buffer (pH 8.1) solution using Tris and NaTris or sodium Tris (MW = 143.14 g/mol)?50 mL of a sample known to contain Mg+2 was taken and titrated with 0.075 M EDTA standard solution by adding pH 10 buffer and EBT indicator. Since the EDTA consumption is 10.5 mL, which of the following is the amount of magnesium in the sample in ppm? (Mg:24 g/mol) A. 378 B. 302 C. 214 D. 251