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How would azithromycin influence quorum sensing in this bacterium?
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- In Methicillin resistant bacteria, what have the bacteria developed that allows them to stay alive in the presence of Methicillin?How would someone design an experiment to test the role of azithromycin on quorum sensing in Pseudomonas aeruginosa?What advantage do quorum-sensing systems confer onbacterial cells?
- What is the purpose of the negative and positive control in the Disc diffusion assay? Why is it important to use positive and negative controls in microbial experimentation? What does it tell us if the positive control leaves a zone of inhibition but the experimental treatment (penicillin disc) does not?For a TLC experiment, what is the purpose of the pentane extraction of spinach juice? Why wouldn’t we want to spot an aqueous solution onto the silica gel?What is density-gradient centrifugation? What are its advantages and disadvantages in subcellular fractionation?
- Why mightproteorhodopsin make a bacterium such as Pelagibacter morecompetitive in its habitat?Why would it be important for the Kirby-Bauer disc diffusion test to use a standard concentration of the bacterial strain being tested?"what is the reason for forming mucoid colonies in some types of bacteria?"