Why would phages pass through the filters used to separate fragments of bacterial cells after the centrifugation step in the exercise?

Q: How was the process of bacterial transformation discovered?

A: The reception of bare DNA fragments from the external environment and the manifestation of that…

Q: What would you surmise if you examined a Kirby-Bauer assay and found individual colonies of the…

A: Antimicrobial is the efficacy to kill the microbes. Drugs that have antimicrobial properties are…

Q: How do we know that during transduction bacterial cell-to- cell contact is not essential?

A: Transduction is a process of genetic recombination by which a foreign DNA is transferred into a…

Q: How does subcellular centrifugation exactly work? At slower speeds, do the smaller/less dense…

A: Introduction: Subcellular centrifugation is a centrifugation technique that is used in the isolation…

Q: Why are many bacteria considered useful?

A: Bacteria are a sort of organic cell. They establish an enormous area of prokaryotic microorganisms.…

Q: What is a mobilizable plasmid?

A:

Q: How could you isolate mitochondria from liver cells using differential centrifugation?

A: Isolated mitochondria are used to perform several functions. This organelle is used to study…

Q: How does MreB control the shape of a rod-shaped bacterium?

A: The cell growth can be achieved by binary fission and spore formation. There are different proteins…

Q: How can you make bacteria competent?

A: The word 'competence' refers to the ability of a cell to alter its genetics by taking up…

Q: Explain how bacterial conjugation differs fromtransformation and transduction.

A: In genetics, the bacterial conjugation is defined as the process which involves in the transfer of…

Q: Why are Archaea resistant to penicillin?

A: Different types of antimicrobial agents are used to inhibit the growth of bacteria. Specific…

Q: What difficulties does one encounter when trying to differentiate bacteria on the basis of…

A: Bacteria are differentiated based on many different characteristics such as cell shape, nature of…

Q: What would you expect to observe if you were able to “transplant” CreS into a rod-shaped bacterium…

A: CreS refers to the protein Crescentin. It is a protein found in bacteria that is similar to the…

Q: How is bacterial transformation useful to us?

A: Bacterial transformation is the process of horizontal gene transfer where in the bacteria take up…

Q: Describe the process of bacterial transformation, describe the controls and methods you would use to…

A: Transformation is a process in through which Bacteria can take up the foreign DNA.

Q: Based on the results of the Kirby-Bauer test shown below, what can we say about this strain of…

A: In Kirby-Bauer testing, bacteria are put on a plate of solid growth medium and wafers of antibiotics…

Q: What tools are used to transfer bacteria

A: Ans - An inoculation loop is used to transfer bacteria for microbiological culture. Petri dish.…

Q: What is the purpose of bacterial transformation?

A: Transformation is the genetic alteration in a cell by direct uptake and incorporation of exogenous…

Q: What would you conclude if you examined a Kirby –Bauer assay and found individual colonies of the…

A: The Kirby - Bauer method is an universally accepted USFDA approved method commonly used in…

Q: A Hfr bacterium mates with an F- plasmid containing bacterium. What will be the type of bacteria…

A: High-frequency recombination (Hfr) cell is a bacterium that carries a conjugative plasmid ( F…

Q: "what is the reason for forming mucoid colonies in some types of bacteria?"

A: Microscopic organisms develop on strong media as provinces. A settlement is characterized as an…

Q: Please briefly explain the initiation of trancription in bacteria

A: Transcription is the process of copying genetic information from one strand of DNA into RNA. In…

Q: Assume that one counted 67 plaques on a bacterial plate where 0.1 ml of a 10-5 dilution of phage was…

A: Bacteria are minute organisms that cannot be observed with unaided eyes. These organisms inhabit a…

Q: Outline the steps of bacterial transduction?

A: Bacteria are single-celled microorganisms. Some of them are injurious while some are beneficial to…

Q: What advantage do quorum-sensing systems confer onbacterial cells?

A: Quorum sensing is a way of communication between cells. It is a signaling pathway. Quorum sensing is…

Q: Explain in your own words how bacteria are grown to form the sample for the antibiotic resistance…

A: Antibiotic resistance The term antibiotic resistance is made up of two words i.e. resistance(ability…

Q: Briefly explain, using the bacteria's response to environment, why the time =0 minutes is often not…

A: Bacterial growth has four different phases: Lag phase, log phase, stationary phase, and decline or…

Q: What are the steps of bacterial artificial transformation?

A: Transformation of bacteria and certain other cells is a versatile tool in genetic engineering. The…

Q: how does the use of the streak plate technique enables us to isolate and purify bacteria

A: The streak plate technique is the popular method or technique to isolate and purify the bacteria.

Q: In the Griffith experiment, why didn't the bacteria from rough colonies harm the mice? Because they…

A: Bacteria like Streptococcus do not have membrane-bounded organelles, can reproduce individually,…

Q: Can HFr conjuation increase bacterial diverstity

A: Prokaryotes are the single celled organisms (unicellular) and are the simplest form, which do not…

Q: What are the disadvantages of using the mouth in filling a pipette? Why do we use exponential…

A: Introduction: 1. A Pipette is widely used in biochemistry labs as well as in other biology labs,…

Q: In the shown disc diffusion assay, how many antibiotics have no antibacterial effect at all?: Select…

A: The Kirby-Bauer procedure also known as a disk diffusion test is performed to test the antibiotic…

Q: What is required for a bacteriophage T4 virion toattach to an Escherichia coli cell?

A: T4 bacteriophage is the well-studied member of Myoviridae, the most complex family of tailed phages.…

Q: List two methods that can be used to make E. coli cells competent.

A: E Colo is a bacteria that is found in warm blooded organisms. There are some methods to make it…

Q: What is micropropagation?

A: Tissue culture technique involves growing cells or tissues in an artificial medium, which is…

Q: Transformation experiment was first performed on which bacteria?

A: In 1928, Griffith performed an experiment to show us that bacteria can change their function and…

Q: Besides autoinducer synthesis, what intracellular moleculepromotes biofilm formation in many…

A: Autoinducers are produced by the bacterial cells as signaling molecules that act in response to…

Q: What is bacterial conjugation and how was it discovered?

A: Horizontal gene transfer (HGT) is defined as the exchange of genetic material between organisms.…

Q: How would azithromycin influence quorum sensing in this bacterium?

A: The bacteria are communicating with each other by the process of qurum sensing. It helps them to…

Q: . In what ways are bacterial transformations useful to humans? (Give as many specific examples as…

A: Bacterial transformation is among modern methods for producing recombinant DNA, wherein genomes from…

Q: Why do we use an Electron Scanning Microscope to see bacteriophages over other microscopes?

A: Microscopes are laboratory and scientific instrument that are used to enlarge objects and specimens…

Q: What is the practical implication of exponential growth in bacteria?

A: When a broth culture is inoculated with a small amount inoculum, the population size of the bacteria…

Q: What would happen to a bacteria that lost a plasmid for antibiotic resistance?  Would it still be…

A: The term Antibiotic resistance  refers to the phenomenon where the bacteria develops a resistance…

Q: Explain how the minimum generation time for the bacteriumEscherichia coli can be less than the time…

A: Escherichia coli is the gram negative unicellular motile  bacteria that are found in the intestine…

Q: why is Lysozyme treatment useful in bacterial cell lysis when protien isolation is happening.

A: Lysozyme is a enzyme found in many human secretions like tears, saliva etc. that function as…

Q: What technique is used to stain bacteria? Who developed this technique?

A: Microorganisms are such small living organisms that are less than 0.1 mm, and can be seen only under…

Q: How are we to know which of the bacteria have taken up the plasmid?

A: The process by which the bacterial host takes up the plasmid is referred to as transformation. The…

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."