How would the the triple mutations K557R and D591V and K617A alter the overall protein?

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter15: From Dna To Protein
Section15.1: The Connection Between Dna, Rna, And Protein
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The authors state: "Properties of the single mutants K557R, D591V and K617A, a double mutant,
D591V/K617A, and a triple mutant, K557R/D591V/K617A, were evaluated to find an allosteric
binding site for citrate."
The numbers between the letters represent the residue number altered. The letter in front was the
wild type and the letter afterwards is the mutant.
How would the the triple mutations K557R and D591V and K617A alter the overall protein?
O lower the # of charges, increase the pl
O increase the # of charges, increase the pl
O lower the # of charges, lower the pl
O lower the # of charges, increase the pl
O no change in the # of charges, increase the pl
O no change in the # of charges, lower the pl
Transcribed Image Text:The authors state: "Properties of the single mutants K557R, D591V and K617A, a double mutant, D591V/K617A, and a triple mutant, K557R/D591V/K617A, were evaluated to find an allosteric binding site for citrate." The numbers between the letters represent the residue number altered. The letter in front was the wild type and the letter afterwards is the mutant. How would the the triple mutations K557R and D591V and K617A alter the overall protein? O lower the # of charges, increase the pl O increase the # of charges, increase the pl O lower the # of charges, lower the pl O lower the # of charges, increase the pl O no change in the # of charges, increase the pl O no change in the # of charges, lower the pl
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