What is a cavity mutant? Which sites in the L121A/L133A mutants demonstrated higher mobility of the nitroxide label? What is special about these locations? Does this fit with what you know about secondary structure?
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- If this molecule can inhibit toxic effects at 0.5mM against gastric carcinoma cells, does it mean that the dimer of this nucleoside analogue will exhibit an IC50 of about 0.25mM?CYP2E1 has a much higher Km for ethanol than ADH (11 mM versus 0.05 mM,respectively). When greater portions of ethanol are ingested (0.08% or 17 mM), which part of the statement would be true?In a-c below, will the residue on the right-hand side increase or decrease the pKa of the residue on the left-hand side? Explain.
- This molecule forms a dimer via pi interactions, resulting to effective molecular stacking. If the molecule can inhibit toxic effects at 0.5mM against gastric carcinoma cells, does it mean that the dimer of this nucleoside analogue will exhibit an IC50 of about 0.25mM?5 25) Although the UV280 extinction coefficient of BSA is well known, why was the BCA assay employed to measure protein concentration of the folate labeled BSA? Please provide an explanation. (C) Would the use of reducing agents have an affect on the tertiary structure of BSA—please explain your answer (you will need to investigate the structure of BSA beyond this article)?what does the abbrevation PPE refer to?
- Given a tripeptide Cys-His-Lys, Cys: Pk1 = 1.71; Pk2 = 10.78; PkR = 8.33 His: Pk1 = 1.82; Pk2 = 9.17; PkR = 6.0 Lys: Pk1 = 2.18; Pk2 = 8.95; PkR = 10.53 What is the dominant structure at pH 2.0? What is the first buffering region of the tripeptide?What are the biochemical systems found in nuclear fraction, mitochondrial fraction and microsomal fraction? Why do the organelles require different centrifugation sediment rates and time in order for them to be isolated? Explain the differences in the amount of proteins, lipids, carbohydrates and nucleic acids in the hydrolyzed chicken liver extract. What is Sudan IV? Write its structure and chemical name?Given a tripeptide Cys-His-Lys, Cys: Pk1 = 1.71; Pk2 = 10.78; PkR = 8.33 His: Pk1 = 1.82; Pk2 = 9.17; PkR = 6.0 Lys: Pk1 = 2.18; Pk2 = 8.95; PkR = 10.53 draw the protonic equilibria for the tripeptide what is the IpH?
- You have discovered a new hemoglobin variant you are calling Hb-21. You have noticed the following from your studies of Hb-21: - The binding constant of Hb-21 is the same at pH 7.1 and 7.4.- The binding constant curves generated by Hb-21 and O2 are sigmoidal.- The Hill coefficient for Hb-21 is 3.-The binding constant for Hb-21 with O2 in the presence of 5mM 2,3-bisphosphglycerate is about 6-times lower instead of the 8-times lower that is observed under these conditions for the wild type enzyme. Use this information to answer questions: Based on this information which of these features of hemoglobin would you conclude are altered in Hb-21 as compared to the wild type hemoglobin? ____ the cooperativity.____ the amount of T versus R state in the tissues. ____ the Bohr effect. What is the major issue for an individual with the Hb-21 variant? Explain exactly what aspect of hemoglobin’s function is altered.Given a tripeptide Cys-His-Lys, Cys: Pk1 = 1.71; Pk2 = 10.78; PkR = 8.33 His: Pk1 = 1.82; Pk2 = 9.17; PkR = 6.0 Lys: Pk1 = 2.18; Pk2 = 8.95; PkR = 10.53 draw the protonic equilibria for the tripeptide what is the IpH? What is the dominant structure at pH 2.0? What is the first buffering region of the tripeptide?Certain individuals with mild forms ofβ-thalassemia produce, in addition to normal adulthemoglobin with two α chains and two β chains,lower levels of an unusual, so-called Leporehemoglobin with two α chains and two chains ineach of which the N-terminal half comes from anormal δ chain and the C-terminal half comesfrom a normal β chain. Certain other individualswho are asymptomatic produce a different, unusualanti-Lepore hemoglobin that contains two α chainsand two chains in which the N-terminal half comesfrom a normal β chain and the C-terminal halfcomes from a normal δ chain.a. Describe an event that could give rise to bothLepore and anti-Lepore hemoglobins