How would you use a ChIP (chromatin immunoprecipitation) to show that when RNA pol II is bound at the promoter of Gene XYZ it recruits Capping Enzyme (but not PolyA Polymerase), and that when RNA pol II is downstream of the promoter near the polyA signal for Gene XYZ that it recruits PolyA Polymerase (but not Capping Enzyme)? Be sure to show what positive results would look like and indicate what controls you would use. I am looking for a well developed plan with lots of details with an illustration of the ideal autoradiogram, with info on primers, antibodies, etc.
How would you use a ChIP (chromatin immunoprecipitation) to show that when RNA pol II is bound at the promoter of Gene XYZ it recruits Capping Enzyme (but not PolyA Polymerase), and that when RNA pol II is downstream of the promoter near the polyA signal for Gene XYZ that it recruits PolyA Polymerase (but not Capping Enzyme)? Be sure to show what positive results would look like and indicate what controls you would use. I am looking for a well developed plan with lots of details with an illustration of the ideal autoradiogram, with info on primers, antibodies, etc.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Transcribed Image Text:How would you use a ChIP (chromatin immunoprecipitation) to show that when
RNA pol Il is bound at the promoter of Gene XYZ it recruits Capping Enzyme (but
not PolyA Polymerase), and that when RNA pol Il is downstream of the promoter
near the polyA signal for Gene XYZ that it recruits PolyA Polymerase (but not
Capping Enzyme)? Be sure to show what positive results would look like and
indicate what controls you would use. I am looking for a well developed plan with
lots of details with an illustration of the ideal autoradiogram, with info on primers,
antibodies, etc.
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