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- The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67K 32 Uses a lamp and a flame for determining the concentrations of metal ions in solutions. a. High performance liquid chromatography b. gas chromatography c. mass spectrometry d. infrared spectroscopy e. UV/VIS spectroscopy f. atomic absorption spectroscopy g. thin layer chromatographyWhich statement is true? Statement 1: In Thin Layer Chromatography (TLC), the polar compounds will have a greater Rf value compared to that of non-polar compounds.Statement 2: The stationary phase in TLC is polar.
- 1. In thin layer chromatography (TLC), explain how the pigments separate on a piece of paper. 2. If the spots are colorless on a TLC plate, how can you make it visible? Enumerate 2 ways and discuss each.3.Explainthethin-layerchromatographicpuritycheckprocedureforanalysisofanunknownorganic compound.The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67 Group of answer choices II > IV > III > I I > III > IV > II I > II > III > IV IV > II > III > I
- Question: Can thin-layer chromatography (TLC) be used to differentiate between two closely related compounds with similar structures?Describe the following scanning electron microscopy of powder micrographs of CoMass spectrometry and X‑ray diffraction are common biochemical techniques for characterizing proteins. Classify each statement based on whether it applies to mass spectrometry, X‑ray diffraction, or both techniques.
- Which of the following statement is false? a. Component with the strong attraction to the support move more slowly than component with weak attraction. b. Chromatography technique used to separate only colored compounds. c. On TLC, single spot does not mean or guarantee a single substance (compound). d. Iodine vapor and UV- lamp are methods used for visualization uncolored compounds.1. How does chromatography separate the components of leave samples? 2. Can you use chromatography to purify a mixture? why? 3. What is Rf value and its significance in chromatography?10. In what ways can a refractive index be used to characterize a liquid sample? 11. Describe how to adjust the light for measurement in the Abbe Refractometer