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hypothesis: How will the temperature affect the expression of CHL when ribosome stalling is done in vivo?
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- Briefly describe an experiment you could perform to isolate ER-associated ribosomes and test if these are capable of translating mRNAs that code for cytoplasmic proteins.Describe results that could be obtained from ribosomeprofiling that would indicate the existence of aregulatory mechanism operating at the level oftranslational initiation.INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following types of mutants in E. coli. Mutant a: The map position of this mutation is in the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if large amounts of tryptophan are present in the growth medium. Mutant b: The map position of this mutation is in the trp operon. The mutant cells do not produce any enzymes coded for by the trp operon under any conditions. Mutant c: The map position of this mutation is some distance from the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if the growth medium contains large amounts of tryptophan.
- Compare and contrast the formation of mRNA in bacterial and eukaryotic cells. How do the differences affect the way in which each type of mRNA is translated? Does one system have obvious advantage in term of energy cost? Which system offers greater opportunities for control of gene expression?Tell how have microarrays demonstrated that, all cells of anorganism have the same genome, some genes expressed in almostall cells, whereas other genes show cell- and tissue-specific expression?Compare the auto induction media (AIM) with regular induced protein expression. Which is better when conducting experiments?
- Yeast cells are eukaryotes, and they require a functional secretory pathway to grow and to maintain cellular organization. But surprisingly, when SRP is removed from yeast cells by deleting the relevant genes, the cells are still alive (although they grow slowly). a) How can yeast survive without SRP? Propose two alternative hypotheses. b) How might electron microscopy of normal and SRP-deficient yeast cells help you to distinguish between these two hypotheses?Explain why release factors are called “molecular mimics.”Yes or no! Does formaldehyde create covalent bonds between 2 proteins and proteins and nucleic acids? does promoters are DNA sequence that rna polymerase are recruited to start transcription? does NAV use tocreate crosslink between biomolecules! does digoxigenin can be attach to a nucleotide and use to distinguish riboprobe from endogenous?
- Give typing answer with explanation and conclusion to all parts What would happen to the overall process of making proteins (transcription-translation) if the pores in the nuclear envelope were blocked? Q10. Suppose that an mRNA transcript consists of the following sequence of bases: AUGCCAGGUUAUGUCUAG. a. What sequence of amino acids would this translate to? b. Now suppose that a mutation takes place in the DNA so that twelfth base changes from U to G. How does this change the meaning of the 4th amino acid? (I.e., what does it change to?) c. Would the result be a normal protein? EXPLAIN SPECIFICALLY WHY OR WHY NOT.summarize the role of galactose, GAL80, GAL4, GAL3 in relation to upstream activator sequencePredict the outcome of the following hypothetical experiments and be sure to explain and be rational in addition to proposing the probable outcome of the experiment: -> you alter the Rab-GDI binding site so that it cannot interact with Rab-GDP -> you have identified a drug that can specifically deplete PI(4)P from the trans-Golgi of the cell. When you treat cells with this drug you hypothesize.... -> while investigating novel anti-cancer drugs you identify a compound that disrupts y-tubulin localization to the centrosome and disperses it throughout the cytosol. Why might this compound be a useful anti-cancer agent?