I have 20 ml of a bacterial culture. After preparing a serial dilution as shown below and plating 0.2 ml of sub-dilution 3 on a Petri dish, I counted 45 cfu. What was the cell density of my culture? Round to a whole number, do not write in scientific notation.

I have 20 ml of a bacterial culture. After preparing a serial dilution as shown below and plating 0.2 ml of sub-dilution 3 on a Petri dish, I counted 45 cfu. What was the cell density of my culture? Round to a whole number, do not write in scientific notation.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter33: Automated Methods Of Analysis

Section: Chapter Questions

Problem 33.8QAP

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Question

I have 20 ml of a bacterial culture. After preparing a serial dilution as shown below and plating 0.2 ml of sub-dilution 3 on a Petri dish, I counted 45
cfu. What was the cell density of my culture? Round to a whole number, do not write in scientific notation.
Sub-dilution 1: V(stock) = 4 ml, v(media) = 6 ml
Sub-dilution 2: v(d1) = 0.5 ml, v(media) = 9.5 ml
Sub-dilution 3: v(d2) = 4 ml, v(media) = 6 ml
The cell density of my bacterial culture was
cfu/ml. (Round to a whole number)

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