Show the separation pattern of the following DNA molecules on 1 % agarose gel. Justify your results. 5 kbp 5 kbp 5 kbp 5 kb +
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- Using Figures 8.7 and 8.9 as a guide, draw a dinucleotide composed of C and A. Next to this, draw the complementary dinucleotide in an antiparallel fashion. Connect the dinucleotides with the appropriate hydrogen bonds. FIGURE 8.9 The two polynucleotide chains in DNA run in opposite directions. The left strand runs 5 to 3, and the right strand runs 3 to 5. The base sequences in each strand are complementary. An A in one strand pairs with a T in the other strand, and a C in one strand is paired with a G in the opposite strand. FIGURE 8.7 Nucleotides can be joined together to form chains caled polynucleotides. Polynucleotides are polar molecules with a 5 end (at the phosphate group) and a 3 end (at the sugar group). An RNA polynucleotide is shown at the left, and a DNA polynucleotide is shown at the right.How many kilobases of the DNA strand below will code for the protein product?If your DNA extraction were a concentration of 45ng DNA/ug, how would you prepare 500 ul of a 1 ng/ul solution of DNA in water?
- Describe the function of the following reagents used in the DNA extraction procedure?a) Proteinase K b) 5M Nacl c) Isopropanol d) 1X TE BufferExplain how an agarose gel can separate DNA fragments of different lengths.The oligonucleotide d-ATGCCTGACT was subjected to sequencing by Sanger’s dideoxy method, and the products were analyzed by electrophoresis on a polyacrylamide. Draw a diagram of the gel banding pattern obtained.
- During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityYou have a 20 mg/ml of Ethidium bromide stock solution. You need to a final concentration of 2ug/ml into a Agarose solution to visualize DNA. What is the dilution factor? Give typing answer with explanation and conclusionBased on your gel, how big are the DNA fragments in each lane? Enter your results in Table 2.
- What does the 260 nm light detect when measuring the purity of a DNA sample? Please select the single answer that is most correct. DNA RNA Proteins The sugar-phosphate backbone of the DNA The heterocyclic rings of the DNAIn standard agarose gels used for the analysis of DNA, linear DNA fragments are separated on the basis of their... a) Size b) Charge c) Buoyancy d) Color e) SmellYou perform an experiment to determine the complete melting profile of a relatively long DNA duplex with 80% G:C base pairs. Sketch a graph of Absorbance at 260 nm (Y-axis) vs. Temperature (X-axis). Label the location of the Tm and describe what is happening to the DNA structure in each segment of the graph (lower Temps, highest Temps, at Tm).