Explain the effect of alkali on DNA structure and stability
Q: DNA bases can be damaged by all of the following except: a. Water b. UV Light c. Hyroxyl…
A: DNA (deoxyribonucleic acid) is the genetic material of an organism that is inherited from the parent…
Q: Spectroscopy is a useful tool to determine the concentration of DNA in a solution by measuring the…
A: Spectroscopy is a field of analytical biochemistry that enables us to conduct tests on quantitative…
Q: Base nitroɡen DNA pair which are have 2 bond of H₂ inside of them?
A: A nucleotide is formed by nitrogenous base, sugar and phosphate. DNA is made up of polymer of…
Q: According to Chargaff's observations of nucleotide composition of DNA samples OA % of (G + C) + % of…
A: Chargaff's rule has been stated for the DNA, which states that the ratio of purine and pyrimidine…
Q: If a DNA molecule has 33%G calculate the percentages of A, T, and C
A: A DNA is composed of basic 4 nucleotide bases: Adenine (A) Guanine (G) Cytosine (C) Thymine (T)
Q: Explain how an agarose gel can separate DNA fragments of different lengths.
A: Agarose gel electrophoresis is one of the important molecular techniques used for the separation of…
Q: A reaction mixture contains DNA polymerase, the four dNTPs, and one of the DNA molecules whose…
A: A DNA polymerase is a member of a family of enzymes that catalyze the synthesis of DNA molecules…
Q: Consider the structure of double-stranded DNA. When DNA is placed into distilled water, it…
A: Deoxyribonucleic acid (DNA) is defined as an organic chemical that will contain all the genetic…
Q: Describe the characteristics of the extracted DNA, such as colour, shape, size, and consistency.
A: DNA is the genetic material of most organisms and it is more stable than RNA.
Q: The absorbance of a nucleic acid sample at 260 and 280 nm are 0.4857 and 0.2427, respectively. Based…
A: The UV absorbance method works on the idea that nucleic acids (DNA or RNA) have conjugated double…
Q: Using recycled papers and sticks, demonstrate the steps of DNA replication. Use the following…
A: During replication, a double-stranded DNA molecule is duplicated to produce two identical DNA…
Q: In a melting profile for DNA, the absorbance at 260 nm increases as result of the disruption of the…
A: Asked : Reason of Absorbance increases by disruption of DNA structure.
Q: Define deoxyribonucleic acid (DNA)
A: The four most important biomolecules in living things are carbohydrates, amino acids, lipids, and…
Q: What is the structure of the gel stain being used? There are several other dyes that can bind DNA…
A: Gel staining is very important visualization and detection procedure that follows protein…
Q: How Can Fragments of DNA Be Separated From One Another? Agarose gel electrophoresis is a procedure…
A: Note- as we are allowed to only 3 subparts of a question, i can provide answers for first three…
Q: Explain in details the influence of ions and water (solvent) in stabilizing DNA structure
A: The presence of phosphate groups in the nucleotides makes the DNA negatively charged. Hence, the…
Q: The DNA extract in this experiment is considered to be “crude” since it has not been separated from…
A: Cellular proteins which are bound to the DNA is removed by adding a protease, or it is removed by…
Q: Match each DNA component to its corresponding point of attachment. 1. carbon 3' 2. carbon 5' 3.…
A: Deoxyribonucleotide (DNA) is a molecule containing all the genetic information needed to make each…
Q: IT IS POSSIBLE TO ESTIMATE THE CONCENTRATION OF SPECIFIC DNA SEQUENCE IN A SOLUTION OF MIXED DNA.…
A: DNA can be quantified through nanodrop with a particular absorbance.
Q: AKS 5a: Which of the following combinations is true of the nucleotide composition of a sample of…
A: According to Chargaff's rule, DNA of organisms should have 1:1 stoichiometric ratio of pyrimidine…
Q: Describe the excision repair process in DNA, using the excision of thymine dimers as an example.
A: DNA (Deoxyribonucleic Acid) It is defined as a genetic material which has all the stored genetic…
Q: The oligonucleotide d-ATGCCTGACT was subjected to sequencing by Sanger’s dideoxy method, and the…
A: Electrophoresis is a technique that separates the molecules basing on the differences in the charges…
Q: Propose an experiment that would test the importance of ionic strength in the determination of DNA…
A: The DNA melting point is the temperature at which DNA helices are denatured and it is referred to as…
Q: -rich DNA is denser than -rich DNA because has a higher molecular mass than while occupying the same…
A: DNA is made up of nitrogenous bases (adenine, guanine, cytosine, and thymine). DNA is usually a…
Q: IF A DOUBLE-STRAND DNA SAMPLE WAS COMPOSED OF 10 PERCENT THYMINE, WHAT WOULD BE THE PERCENTAGE OF…
A: The deoxyribonucleic acid is the genetic material that is passed from one generation to the next…
Q: Similarities between DNA agarose gels and SDS-PAGE are:
A: Gel electrophoresis is the technique of separation of molecules of the basis of their size and…
Q: The DNA extract in this experiment is considered to be “crude” since it has not been separated from…
A: DNA is our genetic material and have been subjected for studies . it is important to extract pure…
Q: In DNA and plasmid extraction using the kit method, silica membrane is used. How could the nucleic…
A: Silica based DNA extraction method is an easy and economical method for extracting nucleic acid. It…
Q: Discuss the reasons proteins were generally favored over DNA as the genetic material before 1940.…
A: Genetic material is either DNA for all normal cella and RNA for viruses only. These materials are…
Q: what happens to the intactness of DNA if extracted DNA fibers were placed in buffer of pH 3?
A: Extracted DNA is generally stored in neutral pH.
Q: Explain the principle involved in the following chemical tests for the characterization of DNA a.…
A: DNA is deoxyribo nucleic acid that is chemically composed of deoxyribose Sugar, nitrogen base and…
Q: Adenine and thymine are complementary base pairs. The two strands are anti-parallel. The base…
A: DNA are the molecule that carries genetic information in all living things.
Q: 1 2 3 4 M 1000 b 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp
A: The diagram is showing the separation of DNA fragments using gel electrophoresis. In-gel…
Q: During agarose gel electrophoresis, why does DNA move through the gel when electric current is…
A: Gel electrophoresis is basically a method that aid in separating DNA fragments and other molecules…
Q: With regard to Chargaff’s experiment described in Figure shown,answer the following:A. What is the…
A: Erwin Chargaff carried out an experiment to determine the base composition of DNA. He concluded that…
Q: Match the following terms with their correct definition. The structure of double-standed DNA Hold…
A: Monomers are the simpler units which joins to each other to make polymers. There are many polymers…
Q: Why evenly spaced sequence in chromatogram is an indication of a good DNA chromatogram?
A: The characteristics of an evenly spaced sequence are:- Evenly-spaced peak, each being…
Q: Give 3 strand slippage and trinucleotide repeat disorder examples
A: DNA is the source of genetic information. It transfers genetic information from parents to…
Q: Explain how to prepare 2 ml of a solution with a concentration of 1 μg/5ml from a stock solution of…
A: Given- Stock solution of DNA sample- 0.1 mg/ml Volume required= 2 ml Concentration of the DNA stock…
Q: Identify the relationship between the size of the DNA and the structure of the agar gel that…
A: Gel Electrophoresis is defined as the process in which the DNA is run under the influence of an…
Q: 1.3 Considering the atomic structure of DNA shown below, what are the features of the molecule that…
A: Introduction: DNA stands for 'deoxyribonucleic acid' and it is the hereditary material in humans and…
Q: Show the separation pattern of the following DNA molecules on 1 % agarose gel. Justify your results.…
A: Introduction: Agarose gel electrophoresis is a molecular biology technique to analyze and separate…
Q: Give one more example of chemical (besides acids and alkalis) which can also affect DNA stability.…
A: DNA is important as it carries all the cell's information and the hereditary information. This makes…
Q: You are given a tube containing 1.5 ml of a DNA solution. The concentration of the DNA solution is…
A: The nucleus of a cell contains the nucleic acids which are responsible for the passing down of…
Q: Define which is the suitable pH to promote binding of DNA to the magnetic beads ?
A: A gene is a fundamental unit of heredity and a succession of nucleotides in DNA or RNA that encodes…
Q: II. Phoebus Levene (1920s) He is perhaps best known for his incorrect tetranucleotide hypothesis of…
A: Phoebus Levene is an American chemist and best known for his work on nucleic acids. He discovered…
Q: In analyzing the base composition of a DNA sample, a student loses the information on pyrimidine…
A: Nucleotides are defined as the organic molecule that acts as the basic building block of the DNA and…
Q: In analyzing the base composition of a DNA sample, a student loses the information on pyrimidine…
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Explain the effect of alkali on DNA structure and stability
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- Why only distilled water used as a solvent for the DNA molecules? What is the characteristic of the molecule that makes it soluble to water? In 5 sentencesExplain in details the influence of ions and water (solvent) in stabilizing DNA structureDefine which is the suitable pH to promote binding of DNA to the magnetic beads ?