Question

Are the bands indicated in Step 6 (SDS-PAGE) likely visualized by western blot or by a general protein stain (such as Coomassie or silver)? 

STEP 1
YFP
Trarsfect into ouilts
STEP 2
Epitope-tagged protain
(ancoded by eDNA)
STEP 3
Lyse calls
YFP
Competitive eluion with
peptide encoding the epitope
YFP Gel elactrophoresis
A
YFP A
B
STEP 5
STEP 6
Afinty-based purification uang
immobilized artibody against the epitape
STEP 4
Excise bands end analyze
by mass spactrometry
STEP 7
Expand
Transcribed Image Text

STEP 1 YFP Trarsfect into ouilts STEP 2 Epitope-tagged protain (ancoded by eDNA) STEP 3 Lyse calls YFP Competitive eluion with peptide encoding the epitope YFP Gel elactrophoresis A YFP A B STEP 5 STEP 6 Afinty-based purification uang immobilized artibody against the epitape STEP 4 Excise bands end analyze by mass spactrometry STEP 7

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