In a clinical analysis laboratory, the level of sodium in the blood is determined by atomic emission spectrometry. The method uses lithium as an internal standard, since the sample treatment is very complex and can cause ion losses. Also, the lithium signal does not interfere with that of sodium. The table below shows the results obtained after measuring different sodium standards to which a constant concentration of lithium (100 mM) has been added. Na concentration (mM) Na signal (UA) Li signal (UA) 0 0 0,180 20 0,036 0,220 50 0,066 0,160 100 0,125 0,190 150 0,200 0,210 200 0,315 0,250 a) Calculate the calibration line corresponding to the internal standard method. b) A blood sample was analyzed with said method and the following signals were obtained: 0.197 AU for sodium and 0.23 AU for lithium. What was the sodium concentration of the tested blood?
In a clinical analysis laboratory, the level of sodium in the blood is determined by atomic emission spectrometry. The method uses lithium as an internal standard, since the sample treatment is very complex and can cause ion losses. Also, the lithium signal does not interfere with that of sodium. The table below shows the results obtained after measuring different sodium standards to which a constant concentration of lithium (100 mM) has been added.
|
Na concentration (mM) |
Na signal (UA) |
Li signal (UA) |
|
0 |
0 |
0,180 |
|
20 |
0,036 |
0,220 |
|
50 |
0,066 |
0,160 |
|
100 |
0,125 |
0,190 |
|
150 |
0,200 |
0,210 |
|
200 |
0,315 |
0,250 |
a) Calculate the calibration line corresponding to the internal standard method. b) A blood sample was analyzed with said method and the following signals were obtained: 0.197 AU for sodium and 0.23 AU for lithium. What was the sodium concentration of the tested blood?
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