In a spectrophotometry experiment, (34.1±0.2)% of the light (=I/Io) was transmitted through the sample. The molar absorptivity, e, of the analyzed was measured separately and determined to be (1.47±0.03)×104M' cm', and the path length of light, b, was (1.00 + 0.01) cm. Determine what is the concentration and the uncertainty in concentration of the analyte, with proper units. Be sure to show your work. In this problem you will need to use Beer's Law.

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter1: Introduction
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Styles Styles
Pane
Dicm
6
In a spectrophotometry experiment, (34.1±0.2)% of the light (=I/lo) was transmitted through the
sample. The molar absorptivity, e, of the analyzed was measured separately and determined to be
(1.47±0.03)×104M' cm', and the path length of light, b, was (1.00
+ 0.01) cm. Determine what is the concentration and the uncertainty in concentration of the
analyte, with proper units. Be sure to show your work. In this problem you will need to use
Beer's Law.
Transcribed Image Text:Styles Styles Pane Dicm 6 In a spectrophotometry experiment, (34.1±0.2)% of the light (=I/lo) was transmitted through the sample. The molar absorptivity, e, of the analyzed was measured separately and determined to be (1.47±0.03)×104M' cm', and the path length of light, b, was (1.00 + 0.01) cm. Determine what is the concentration and the uncertainty in concentration of the analyte, with proper units. Be sure to show your work. In this problem you will need to use Beer's Law.
e oiar absorptivity, ɛ, of the analyzed was mea
±0.03)×10ª M-' cm', and the path length of lig
e what is the concentration and the uncertainty in
its. Be sure to show your work. In this problem
-1
A = –log10 ()
-log10 2.303
=,207 In (-)
ɛbc
ed to reduce the uncertainty in your final result, w
ments should you focus on making better? Or equi
nty in fraction of light absorbed, uncertainty in mo
oth) cotributes most to the overall uncertainty in t
Transcribed Image Text:e oiar absorptivity, ɛ, of the analyzed was mea ±0.03)×10ª M-' cm', and the path length of lig e what is the concentration and the uncertainty in its. Be sure to show your work. In this problem -1 A = –log10 () -log10 2.303 =,207 In (-) ɛbc ed to reduce the uncertainty in your final result, w ments should you focus on making better? Or equi nty in fraction of light absorbed, uncertainty in mo oth) cotributes most to the overall uncertainty in t
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-log10II0=εbcc=-log10II0εb

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