The following instrumental readings were obtained for the determination of low concentrations of sodium by flame photometry. 175, 104, 164, 193, .131, 189, 155,133, 151 and 176. Ten blanks had a mean reading of 45.O. The slope of the calibration curve is 1.75x10 M :Answer the following questions (-1) Mean concentration of sodium (X Standard deviation of the sodium measurement Signal detection limit iv) Lowest detection limit in umole
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- You are developing a procedure for determining traces of copper in biological materials using a wet digestion followed by measurements by atomic absorption spectrophotometry. In order to test the validity of the method, you obtain a NIST orchard leaves standard reference material and analyze this material with your procedure five times and obtain a mean of 12.27 ppm with a standard deviation of 0.097 ppm. The NIST sample is listed as 11.7 ppm. Does your method give a statistically valid value at the 95% confidence level?What is the role of stationary phase and mobile phase in Chromatography? Please shortly answer at your own words. Answer should be to the point (specific 4-5 lines).The detecton limits reported for Al with ICP-AES and GF-AAS are 2 ppb and 5 picograms, respectivly. If we assume that a 20 microliter sample volume is used for the GF-AAS determination, which method has the lower detection limit? (1 picogram = 10-12 gram.)
- You are developing a Ca detection method for a graphite furnace AA instrument. Your analysis of ten blank samples gives a standard deviation of 0.265 ppb. To establish your limit of detection (LOD), what concentration of Ca should be spiked into your blanks?Concentration=2.5 X 10^3 μM OD400nm=0.859 1cm cuvette is used what is the extinction coefficient (μM^-1 cm^-1)a. What mass of Ag2SO4 is formed when 34 mL of 0.300 M AgNO3 are mixed with 26 mL of 30.0 g/L Na2SO4? b. Potassium can be determined by flame emission spectrometry (flame photometry) using lithium internal standard. The following data were obtained for standard solutions of KCI and an unknown containing a constant, known amount of Lil as the internal standard. The intensity of a blank was 0.04. Calculate the concentration of K in the unknown. ck, ppm Intensity of K emission Intensity of Li emission 5.0 34.7 5.8 20.0 110.2 9.1 Unknown 57.3 6.8
- Which sentence is false about gravimetric analysis? a. It is used for inorganic b. It is used to assay barium c. It is used to assay of d. Relative precision 3% to 4%A graphite furnace atomic absorption spectrophotometer (AAS) was used to determine the arsenic (As) concentrations in drinking water. A large set of blank samples yielded an average blank signal of 0.013 and a blank standard deviation of 0.0030. The following data were obtained for a calibration curve. [As] (ppm) 2.0 4.0 6.0 8.0 10.0 Absorbance 0.17 0.28 0.48 0.53 0.72 Using a spreadsheet on an Excell, create a graph of the data using symbols only for the data points. Plot the line on the same graph and include the graph to this question. (a). What is the sensitivity of the method? (b). Calculate the LOD and LOQ for As. (c). A sample of water yielded an absorbance of 0.43. What is the concentration of As in the water?In quality management, it is very important that you have a good background knowledge in both descriptive and inferential statistics. Since you already took up Biostatistics and Epidemiology during your Second Year, answer BRIEFLY the following questions on basic statistics: 1. Compare and contrast the three most common measures of central tendency mean, median and mode. 2. Differentiate standard deviation from coefficient of variation. 3. What is T-test?
- The following results were obtained for the determination of calcium in a NIST limestone sample: %CaO = 50.33, 50.22, 50.36, 50.21, and 50.44. Five gross samples were then obtained for a carload of limestone. The average %CaO values for the gross samples were found to be 49.53, 50.12, 49.60, 49.87, and 50.49. Calculate the relative standard deviation associated with the sampling stepA researcher was attempting to quantify the amount of dichlorodiphenyltrichloroethane (DDT) in spinach using gas chromatography mass spectrometry (GCMS). First attempts using external calibration proved difficult, as reproducibility and external standard agreement were poor. To overcome these problems, the researcher used the internal standard calibration method with chloroform as the internal standard. To begin, the researcher analyzed a sample containing 5.67 mg/L DDT standard and 1.84 mg/L chloroform as the internal standard, which produced peak areas of 4035 and 10177 for the DDT and chloroform, respectively. After establishing the response factor of the instrument, the researcher collected 11.53 g of spinach, homogenized the sample, and extracted the DDT using an established method, producing a 3.70 mL solution containing an unknown amount of extracted DDT. The researcher then prepared a sample for analysis that contained 1.00 mL of the unknown DDT solution and 2.00 mL of 10.25…Use the following information collected from a SIZE-EXCLUSION CHROMATOGRAPHY experiment to answer the questions below. MW Standards Molecular Weight (kDa) Elution Volume (mL) blue dextran 1000 85 myoglobin 17.2 200 TIM 53.3 178 hemoglobin 62.2 156 IgG 140 122 ATCase 307.9 94 Prepare a graph showing the linear relationship between log function of molecular weight against Ve/Vo. The R2value must be 0.9 or higher. What is the linear equation from the standards given? What is the R2value?