In a whole-cell patch-clamp experiment at 32 degrees C, the intracellular solution contains 16 mM NaCl, 150 mM K-gluconate, and 1µM Ca(NO3)2, and the extracellular solution contains 150 mM NaCl, 3 mM KCI, 25 mM NaHCO3, 2 mM MgCl2, and 1.5 mM CaCl2. The below graph is a current-voltage (I-V) curve generated by measuring the current fluy thrOUgh a spocific jon chappel over

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
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Chapter22: Bulk Electrolysis: Electrogravimetry And Coulometry
Section: Chapter Questions
Problem 22.28QAP
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In a whole-cell patch-clamp experiment at 32
degrees C, the intracellular solution contains
16 mM NaCl, 150 mM K-gluconate, and 1µM
Ca(NO3)2, and the extracellular solution
contains 150 mM NaCl, 3 mM KCI,, 25 mM
NaHCO3, 2 mM MgCl2, and 1.5 mM CaCl2.
The below graph is a current-voltage (I-V)
curve generated by measuring the current
flux through a specific ion channel over a
range of voltages (-100 to +40mV).
What is the reversal potential of this current?
1000 -
800
600
400-
200
-100 -80
-60
-40
-20
20
40
-200
mv
-400
pA
Transcribed Image Text:In a whole-cell patch-clamp experiment at 32 degrees C, the intracellular solution contains 16 mM NaCl, 150 mM K-gluconate, and 1µM Ca(NO3)2, and the extracellular solution contains 150 mM NaCl, 3 mM KCI,, 25 mM NaHCO3, 2 mM MgCl2, and 1.5 mM CaCl2. The below graph is a current-voltage (I-V) curve generated by measuring the current flux through a specific ion channel over a range of voltages (-100 to +40mV). What is the reversal potential of this current? 1000 - 800 600 400- 200 -100 -80 -60 -40 -20 20 40 -200 mv -400 pA
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