Name at least one reason that we might want to use a Winogradsky column rather than a laboratory medium such as nutrient agar or beef heart agar. Describe why metabolism in the presence of oxygen might cause problems for microorganisms.
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- Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?
- give one reason why is it necessary to keep stirring the agar medium mixture in a beaker when heating in a hotplate?how do microorganisms such as bacteria eliminate harmful reactive oxygen species? Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.Why it is possible/ applicable to use recrystallization and vacuum filtration with Luminol? Cite some reasons.(Explain in at least 1 paragraph)
- Two cultures of a facultative anaerobe are grown in the same medium, but one culture is exposed to air and the other is maintained under anaerobic conditions. Which of the two cultures will contain more cells at the end of a week? Why?Compare and contrast oxygen requirements for obligate anaerobes and microaerophiles. Describe one laboratory media that can cultivate microbes with various oxygen requirements. Include the chemistry and how obligate anaerobes and microaerophiles would look if grown on this media.If a biotechnologist wishes to obtain a primary metabolite, they will harvest the organism/media during log phase. If a biotechnologist wishes to obtain a secondary metabolite, they will harvest the organism/media during stationary phase. Can you explain this distinction regarding the metabolism of the cell at these two different batch culture growth phases?
- Aside from Paraffin, what is your recommended infiltrating medium for routine purposes? why?Answer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in culture media preparation? How does cotton plug prevent contamination of the medium? Why should caps of glassware be loosened first before sterilization?What type of media components cannot be sterilized in an autoclave?