Answered: In eukaryotes, RNA primers are…

In eukaryotes, RNA primers are primarily removed by a. DNA polymerase I. b. DNA polymerase α. c. flap endonuclease. d. helicase

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

See similar textbooks

Related questions

Q: Next generation sequencing using Illumina platforms identifies individual bases in a DNA fragment…

A: The following steps are involved in next generation sequencing: 1) First the DNA sequencing…

Q: You have extracted a long piece of DNA from a human cell and you want to extract the gene of…

A: Genes are a series of instructions that govern how an organism looks, how it survives, and how it…

Q: To synthesize DNA, what does telomerase use as a template? a. It uses the DNA in the 3′ overhang…

A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…

Q: Compare and contrast the properties of the enzymes DNA polymerase I and polymerase III from E. coli.

A: Replication is a process, which involves the synthesis of DNA from the template DNA, with the help…

Q: A DNA microarray is a slide that is dotted witha. mRNAs from a sample of cells.b. fluorescently…

A: Bio-informatics is the branch of biology that uses computers and statistics to analyse and record…

Q: About transcriptomics and genomics, answer: 4. Can we replicate RNA? How can we use PCR and Sanger…

A: (a)RNA viruses can replicate their RNA. RNA can store and replicate genetic information. RNA…

Q: Describe the main steps in using the polymerase chain reaction to amplify a small amount of DNA.

A: Polymerase chain reaction or PCR is a technique which has been used widely to produce multiple…

Q: Which of the following is an example of a recombinant DNA molecule? O a. a PCR-amplified…

A: Recombinant DNA technology : In this process two DNA molecules are attaches with each other for…

Q: For the chromatogram below, what is the sequence of the template DNA from base 115 to 125?…

A: According to the question, we have to answer the question that which is for the chromatograph below,…

Q: Describe What are the sticky ends of the restriction fragments? Select one: a. The surfaces of…

A: Restriction endonuclease are enzymes that help divide DNA and generate small pieces in molecular…

Q: You are trying to clone a gene, You have successfully isolated it from the genomic DNA of an…

A: The term DNA cloning is associated with a process that plays an important role in making copies of a…

Q: A linear plasmid contains only two genes, which are transcribed in opposite directions, each one…

A: There are two forms of plasmid known. One is circular and another is linear. The circular plasmid is…

Q: Advantages of using the PCR include the following EXCEPT A it will always give the desired product…

A: Introduction PCR:- It is a technique to make many copies of a specific DNA region in vitro, It…

Q: a) What dipeptide is produced from the following segment of DNA: AGAGAT? (b) What happens to the…

A: Gene expression is the process in which information stored in the DNA is converted into the…

Q: (a) Why can there be multiple codons for an amino acid? Why would this have evolved? (b) What is…

A: The genetic code comprises the set of rules that tells how the four nucleotide bases in the DNA are…

Q: Explain how DNA polymerase III adds dNTPs onto the RNA primer.

A: DNA Polymerase: A DNA polymerase is an enzyme that catalyses the manufacture of DNA molecules from…

Q: Using the DNA strand shown here as a template, what will be the sequence of the RNA transcript? 5'…

A: A template is the strand that attach to the complementary strand via DNA polymerase and RNA…

Q: Which of the following does not occur during telomerase extension of parental DNA? a. Translocation…

A: Introduction: Telomerase, an enzyme having RNA template, extends its ends by copying the RNA…

Q: in Cohen-Boyer's recombinant DNA procedure _______ must be used for both the bacterial DNA and the…

A: Introduction Cohen and Boyer made recombinant DNA molecules by using inserting a gene of interest…

Q: Which of the following are shared between RNA polymerase and RNA-dependent RNA polymerases? (select…

A: Ans- a) Uses nucleoside triphosphates to build a polymer. e) uses a single-stranded template b)…

Q: a. Why do bacteria make restriction endonucleases? b. What is it about the endonucleases that…

A: Restriction enzymes cleave DNA at or close unique recognizing sequences known as restriction sites,…

Q: A collection of recombinant vectors that carry fragments of chromosomalDNA is calleda. a genomic…

A: Recombinant vectors are generally some live replicating virus particles which carry some extra genes…

Q: The dideoxynucleosides ddATP, ddTTP, ddGTP, and ddCTP were important in DNA sequencing because they…

A: Dideoxynucleotides are DNA polymerase chain-elongating inhibitors used in the Sanger technique of…

Q: Describe an experimental approach to determining the processivity of a DNA polymerase (i.e., the…

A: Processivity is the average number of bases a polymerase enzyme will extend and incorporate base…

Q: Consider a partial restriction digestion, in which genomic DNA is exposed to a small, limiting…

A: The process of cutting or cleaving DNA by using restriction enzymes is called digestion or…

Q: the most efficient general strategy for whole genome sequencing is ? (a) double the coding sequence…

A: Whole-genome sequencing abbreviated as WGS is a method used to comprehensively analyze the entire…

Q: Explain why DNA polymerase requires a template and a primer.

A: DNA replication is a process, in which the DNA polymerase (DNA pol) is the main key proteins that…

Q: Which enzyme is responsible for unwinding and separating the parental DNA strands? a polymerase…

A: Each parental strand provides a blueprint for the synthesis of a new complimentary daughter thread…

Q: Describe the possible outcome of a PCR experiment in which (a) there is a single-stranded break in…

A: The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment…

Q: The experimental insertion of a fragment of frog DNA into a bacterial plasmid produces a(n) ________…

A: Restriction enzymes are DNA-cutting enzymes. Each enzyme recognizes one or a few target sequences…

Q: What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA…

A: Your question contains multiple sub-parts, and as per our policy, we only answer the first three…

Q: When cloning a piece of DNA, the purpose of using the LacZ blue-white colony method is to A Remove…

A: Blue-white screening is a quick and efficient method for the identification of recombinant bacteria.…

Q: For the chromatogram below, what is the sequence of the template DNA from base 115 to 125? CTGTGTGAA…

A: DNA is formed of four types of nitrogenous bases, which are adenine, thymine, guanine, and cytocine.…

Q: Compare DNA polymerase and RNA polymerase from E. coli in regard to each of the following features:…

A: Introduction: DNA polymerases are the group of enzymes that catalyze the synthesis of DNA strands…

Q: During cloning, the DNA is cut with a restriction enzyme giving it what?

A: Answers: 1 - B 2 - A 3 - B 4 - B

Q: Restriction enzymes cut double-stranded DNA _____. a. at noncoding areas between genes b.…

A: Restriction enzymes cut double-stranded only at specific sequence of the dna, producing either a…

Q: You are trying to clone a gene. You have successfully isolated it from the genomic DNA of an…

A: Gene cloning is a process to form multiple copies of desired DNA of interest. This is done to- * to…

Q: These highly polymorphic molecular markers are useful in DNA fingerprinting: (a) plasmid vectors (b)…

A: DNA typing is a laboratory procedure for detecting normal variations in a DNA sample…

Q: b) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis…

A: DNA digestion is the process in molecular biology which is done by restriction enzymes for the…

Q: Define and indicate the significance of (a) Okazaki fragments,(b) DNA ligase, and (c) primer RNA…

A: Introduction DNA replication is very crucial for the continuation of life as every new daughter…

Q: The short Okazaki fragments are Select one: a. spliced together by DNA ligase b. glued together by…

A: Replication is the process of synthesis of daughter stand from the parental strand by the action of…

Q: a. What is the purpose of molecular cloning?b. What purpose do selectable markers serve in…

A: Plasmids are small, circular extrachromosomal DNA present in a cell. These extrachromosomal…

Q: You want to propagate large amounts of a DNA fragment. To do this, use a plasmid vector. a) How…

A: a) DNA cloning is a technique that makes many identical copies of a piece of DNA, such as a gene. In…

Q: (a) Why the plasmid (containing the foreign DNA), together with the competent bacterial cells are…

A: The purpose of molecular cloning is to make numerous copies of a recombinant DNA molecule, and…

Q: PCR

A: Polymerase chain reaction is known as PCR. PCR is a technique which is used to amplify or copy the…

Q: Part A: Antibiotic resistance is a major health concern. Resistance to various antibiotics can be…

A: When the existence of antibiotics and antifungals forces bacteria and fungi to adjust, antimicrobial…

Concept explainers

Tissue regeneration & repair

Tissue regeneration involves the restoration of tissue components by regrowth of damaged tissues. In contrast, tissue repair involves patching of injured tissue rather than restoring it. It is the proliferative capacity of the cells that decides the amount of regeneration against the repair that would occur in the tissue. Both regeneration and repair depending on the duration of the injury and response to the inflammation.

Question

In eukaryotes, RNA primers are primarily removed by

a. DNA polymerase I.

b. DNA polymerase α.

c. flap endonuclease.

d. helicase

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

What would be the result if an organism’s telomerase were mutated and nonfunctional? a. No DNA replication would take place. b. The DNA polymerase enzyme would stall at the telomere. c. Chromosomes would shorten with each new generation. d. RNA primers could not be removed.
the most efficient general strategy for whole genome sequencing is ? (a) double the coding sequence after sequencing the proteins (b) shotgun sequence and assemble based on overlaps (c) identify mutations that affect glycolysis (d) obtain recombinant DNA clone maps before starting the sequencing (e) obtain comprehensive SNP maps before determining the order of DNA clone
Topoisomerases (a) synthesize DNA (b) synthesize RNA primers(c) join Okazaki fragments (d) break and rejoin DNA to reduce torsional strain (e) prevent single DNA strands from joining to form a double helix
A key difference between the original Holliday model and the double-strand break model is the way thata. the DNA strands are initially broken.b. branch migration occurs.c. a heteroduplex is formed.d. resolution occurs.
Okazaki fragments are ________. A. short RNA primers needed for initiation of polymerization B. fragments of DNA polymerase I that lack 5' → 3' exonuclease activity C. short stretches of DNA formed on the lagging strand D. the smallest subunits of DNA polymerase III
The polymerase chain reaction uses Taq polymerase rather than a DNA polymerase from E. coli, because Taq polymerasea) introduces fewer errors during DNA synthesis.b) is heat-stable.c) can initiate DNA synthesis at a wider variety of sequences.d) can denature a double-stranded DNA template.e) is easier to obtain.
Match the E. coli mismatch repair enzyme on the left with the appropriate function on the right. MutS MutH MutL a. facilitates the looping of the region of DNA to be replaced b. distinguish between parental and newly-synthesized DNA c. locates mismatches in the DNA
Base analogs are mutagenic because of which characteristic? a. They produce changes in DNA polymerase that cause it to malfunction. b. They distort the structure of DNA. c. They are similar in structure to the normal bases. d. They chemically modify the normal bases.
Restriction enzymes found in bacterial cells are ordinarily useda. during DNA replication.b. to degrade the bacterial cell’s DNA.c. to degrade viral DNA that enters the cell.d. to attach pieces of DNA together.
Topoisomerases are enzymes that can: a. join two DNA fragments to become one. b. catalyze conformational change of a protein. c. cut DNA at specific site. d. catalyze the breaking and rejoining of DNA strands which produces DNA that is either more or less superhelical than the original.
You want to propagate large amounts of a DNA fragment. To do this, use a plasmid vector. a) How should this plasmid vector be constructed? b) Also describe the function of the components.
DNA Polymerase holoenzymes used for DNA replication recognizes A. double-stranded sequences as starting points B. methylated lipids as start points C. acetylated lipids as start points D. single stranded sequences as starting points