In sheep DNA, the mol % of adenine (A) was found to be 28.3. Based on Chargaff's rule, what would be the approximate mol % of G, C, and T? mol % of G = mol % of C = mol % of T =
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- State the properties of the WatsonCrick model of DNA in the following categories: a. number of polynucleotide chains b. polarity (running in same direction or opposite directions) c. bases on interior or exterior of molecule d. sugar/phosphate on interior or exterior of molecule e. which bases pair with which f. right- or left-handed helixThe DNA from sea urchins contains about 32% A and about 18% G. What percentages of T and C would you expect in sea urchin DNA? Explain., The E. coli genome is 4.6 Mbp (mega base-pairs). What is the physical length of the E. coli genome?
- Based on Chargaff’s rules, if a segment of DNA is composed of 20% adenine (A) bases, what is the percentage of guanine (G)?If the length of E.coli DNA is 1.36 mm, calculate the number of base pairs it containsState the properties of the Watson-Crick model of DNA in the following categories: a) number of polynucleotide chains b) polarity (strand direction running same or opposite c) bases on interior or exterior of molecule d) sugar/phosphate on interior or exterior of molecule e) which bases pair with which f) right- or left-handed helix
- Base analysis of DNA from maize (corn) shows it to have 23 mole percent cytosine (moles per 100 moles total nucleotide). What are the percentages of the other three bases?If the length of E.coli DNA is 1.36 mm,can you calculate the number of base pairs in ECOLI?A duplex DNA molecule contains a random sequence of the four nucleotides with equal proportions of each. What is the average spacing between consecutive occurrences of the sequence 5'-ATGC-3'? Between consecutive occurrences of the sequence 5'-TACGGC-3'?
- By average, how many Sau3A (5’GATC3’) sites are there in a 10 kd DNA molecule? (1/4)^6 * 10,000 = 2.44140625 => 2.4 For the above 10 kb DNA, if you use Sau3A to do a complete digestion, you will only get a lot of small fragments ( 200~500 base pairs). What should I do if I want to get larger digested fragments (say 3 to 5 kb) Please answer asap and type your answer and do not copy from anywhere pleaseLet’s assume the linker region of DNA averages 54 bp in length. How many molecules of H2A would you expect to find in a DNA sample that is 46,000 bp in length?If an RNA sample were composed of 20% adenine, what would be the percentage of guanine?
