In yeast, LYS5, ADE1, and URA2 are genes required to synthesize lysine, adenine and uracil, respectively. A MATa lys5 ADE1 URA2 haploid was mated to a MATA LYS5 ade1 ura2 haploid to make a triply heterozygous diploid. This diploid was put through meiosis, 100 tetrads were dissected and the spore colonies transferred to medium lacking either lysine, adenine or uracil to determine which spores were prototrophic or auxotrophic for those nutrients. The following results were obtained: LYS5-ADE1 ADE1-URA2 LYS5-URA2 PD 20 18 50 NPD I 58 62 42 22 20 8

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In yeast, LYS5, ADE1, and URA2 are genes required to synthesize lysine,
adenine and uracil, respectively. A MATa lys5 ADE1 URA2 haploid was mated
to a MATA LYS5 ade1 ura2 haploid to make a triply heterozygous diploid. This
diploid was put through meiosis, 100 tetrads were dissected and the spore
colonies transferred to medium lacking either lysine, adenine or uracil to
determine which spores were prototrophic or auxotrophic for those nutrients.
The following results were obtained:
LYS5-ADE1
ADE1-URA2
LYS5-URA2
PD
NPD
Ι
20
22
58
18
20
62
50
8
42
Based on this information, select the statements below that are TRUE.
Select 4 correct answer(s)
A) The LYS5 and URA2 genes are linked.
B)
The genotypes of the four spores in the ADE1-URA2 tetratypes is
ade1 ura2
ade1 URA2
ADE1 ura2
ADE1 URA2
C) The map distance between ADE1 and URA2 is 51 CM.
D) ADE1 and URA2 are likely located on the same chromosome.
E) The map distance between LYS5 and URA2 is 29 CM.
☐ F) LYS5 and ADE1 independently assort during meiosis.
G)
The genotypes of the four spores in the LYS5-URA2 NPDS are:
lys5 URA2
lys5 URA2
LYS5 ura2
LYS5 ura2
H) LYS5 and URA2 are likely located on the same chromosome.
Transcribed Image Text:In yeast, LYS5, ADE1, and URA2 are genes required to synthesize lysine, adenine and uracil, respectively. A MATa lys5 ADE1 URA2 haploid was mated to a MATA LYS5 ade1 ura2 haploid to make a triply heterozygous diploid. This diploid was put through meiosis, 100 tetrads were dissected and the spore colonies transferred to medium lacking either lysine, adenine or uracil to determine which spores were prototrophic or auxotrophic for those nutrients. The following results were obtained: LYS5-ADE1 ADE1-URA2 LYS5-URA2 PD NPD Ι 20 22 58 18 20 62 50 8 42 Based on this information, select the statements below that are TRUE. Select 4 correct answer(s) A) The LYS5 and URA2 genes are linked. B) The genotypes of the four spores in the ADE1-URA2 tetratypes is ade1 ura2 ade1 URA2 ADE1 ura2 ADE1 URA2 C) The map distance between ADE1 and URA2 is 51 CM. D) ADE1 and URA2 are likely located on the same chromosome. E) The map distance between LYS5 and URA2 is 29 CM. ☐ F) LYS5 and ADE1 independently assort during meiosis. G) The genotypes of the four spores in the LYS5-URA2 NPDS are: lys5 URA2 lys5 URA2 LYS5 ura2 LYS5 ura2 H) LYS5 and URA2 are likely located on the same chromosome.
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