Interpret the level of gene expression of the lac operon, as well as the status of the lac repressor and the CAP protein, when bacterial growth media contain the following sugar: (i) Lactose but no glucose. (ii) Glucose and no lactose.

Interpret the level of gene expression of the lac operon, as well as the status of the lac repressor and the CAP protein, when bacterial growth media contain the following sugar: (i) Lactose but no glucose. (ii) Glucose and no lactose.

Name: Interpret the level of gene expression of the lac operon, as well as
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Description: Interpret the level of gene expression of the lac operon, as well as the status of the lac repressorand the CAP protein, when bacterial growth media contain the following sugar:(1)Lactose but no glucose.(ii)Glucose and no lactose.

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 13TYU: INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following...

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INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following types of mutants in E. coli. Mutant a: The map position of this mutation is in the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if large amounts of tryptophan are present in the growth medium. Mutant b: The map position of this mutation is in the trp operon. The mutant cells do not produce any enzymes coded for by the trp operon under any conditions. Mutant c: The map position of this mutation is some distance from the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if the growth medium contains large amounts of tryptophan.
Describe the binding of RNA polymerase, repressors, andactivators to the lac operon when both lactose and glucose are scarce. What is the effect of these scarcities ontranscription of the lac operon?
Draw the structure of lac operon and its action when the following conditions are prevailing in the media: increased glucose, low glucose, higher c-AMP, low c-AMP, higher glucose, and high c-AMP, low glucose and High c-AMP, Higher glucose and higher allolactose
describe the actions of the normal and mutated lac operon in absence of lactose
Predict the level of genetic activity of the lac operon as well as the status of the lac repressor and the CAP protein under the cellular conditions listed in the accompanying table. Lactose Glucose (a) - - (b) + - (c) - + (d) + +
Activation, transcription, and translation of the lac operon willrelease which exact nutrients?
Explain why large amounts of transcript result when a bacterium with a lac Operon is in a medium with no glucose and an abundance of lactose.
Compare and contrast the functional mechanism of the Inducible - Lac operon with the Repressible - Tryp operon.
How does catabolite repression affect expression of the lac operon and how can it lead to diauxic growth? What role do CRP and cyclic AMP play? Predict what would happen metabolically if you grew E. coli up in a medium containing both glucose and lactose.
Explain the role of CAP and cAMP in the regulation of the lac-operon.
By using separate labelled diagrams, describe as detail as possile the actions of the normal and mutated lac operon in absence of lactose.
For each of the following conditions in the Lac Operon, state whether transcription will occur or not and state why. Include all the major actors including glucose, lactose, cAMP and CAP(CPR). Key + (present) - (absent) 1) Glucose-; Lactose- 2) Glucose+; Lactose- 3) Glucose+; Lactose+ 4) Glucose-; Lactose+