Interpretation: > Determine the total number of bacteria per plaque sample and toothbrush head from BHI. Number of colonies on a selected plate CFU/ mL = Total dilution x amount plated Questions: Q1: Which of the 6 BHI plates Results of the plates using the plaque sample -1 10 -2 10 shown would be the one to TMTC TMTC TMTC use as your count for the formula? Q2: Set up the formula and solve for # of CFUS per plaque sample. *238 colonies 24 colonles 2 colonies
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- Miles and Misra serial dilution strategy to determine bacterial density (cfu/ml) and if infection is clinically relevant. urine sample what the result shows, what the result means, your confidence in each result, troubleshooting needed for repeating diagnostic test whether the result is useful for forming your scientific opinion, and whether you accept or reject the result (and your justification for this). thank you!!!Please answer and explain TOPIC: STAINING FECAL SMEARS 1.) What are the advantages of staining fecal smears with permanent stains (namely: Iron hematoxylin stain, Wheatley's trichrome stain, and modified acid-fast stain)? Enumerate more than two and explain.INSTRUCTION: Answer the question properly Do not copy in Google, plagiarize checker will be used. QUESTION: Give specific examples of the ff.: (5 each) a.) gram (+) cocci, b.) gram (-) cocci, c.) gram (+) bacilli, d.) gram (-) bacilli, e.) gram (-) curved rod, f.) gram (-) coccobacilli.
- What situations would result in recollection or rejection of specimen? Determine corresponding solutions to avoid such conditions.Give typing answer with explanation and conclusion Matching -Produce gray-green-yellow frothy bubbly discharge -Produce gray-green white sticky discharge -Produce thick white cottage cheese like discharge -West mount exam shows motile flagellated organisms -KOH prep (Wiff Test) gives Fishy odor -Wet-mount prep shows bacteria coated epithelial clue ells -Most common cause of vaginosis is __________ -Microscopic exam shows budding yeast -Metronidazole is a treatment of choice for both the patient and their sexual partners -Nystatin or clotrimazole can be used as treatment for ______ -Metronidazole is a treatment for the patient only -Also considered as a sexually transmitted diseaseBacterial Growth lab: Each 20-minute interval, absorbance of culture was recorded. When the curve showed plateaus was the absorbance for each culture proportional to the number of bacteria present in the tubes?
- microbial sensivity lab: in the procedure used to test bacterial growth against various temperatures (incubator, room, refrigerator, freezer), why should efforts be made to inoculate each tube with the same number of bacteria?Introduction for the stab slant and surface inoculation.Paraphrase the text below: Series of test tubes were filled with the desired volume of the BSA (0.1, 0.2, 0.3… 1 ml) .PBS was added to this to make the volume of 1 mL. 5 mL of copper reagent was added to all the tubes. After proper mixing, all the tubes were incubated at room temperature for 15 minutes. 1 mL Folin reagent was added to each and mixed properly with the help of vortex mixer and incubated for 20 minutes. The intensity of the colour was then determined spectrophotometrically at 680 nm. The graph was than plotted between optical density and the amount of BSA. Proteins estimation was done for the detection for the amount of proteins present in the sample solutions by the Lowry method
- a. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained) b. What is the microscopy technique and magnification used to obtain this image? c. What is the basic principle of image formation using this microscopy technique? d. What can be observed and concluded from the image of the specimen? e. Are there any potential aberrations present in this specimen image? Describe these and how they may affect interpretation of the result.a. What is the total dilution of Tube #4? Express the answer in exponential format. b. You plated 1 mL of the Tube #4, After incubating, you counted 500 colonies on the plate. What is the concentration of Tube #0? include units. c. How could you change the experiment in part B to get a plate in the countable range? Be specific about any dilution factors and/or plated volumes you would change.Please explain and cite references if possible. Thank you! 1. Explain why stool specimen must not be frozen nor placed in incubators.