3) Your initial E.coli concentration is 1x10° CFU/ml (initial tube). The serial dilutions for each tube vou performed were as follows: 2 mls from initial tube into 198 mls of saline (tube 1), 5 ml from tube 1 into 5 mls of saline (tube 2). And then 0.1 ml from tube 2 into 0.9ml saline in tube 3. You plated 0.1ml on one plate from tube 3. Based on this scheme, how many colonies (CFU) do you expect on that plate? Draw out the dilution scheme described above and show me your answer and complete work. lorada la oi ain doo oiloo inon Viable colony counts of overnight (ON) cultures: Dilutions are total/serial plate dilutions after plating 0.1ml of solution from the indicated dilution tube. Serratia marcescens 102, 104, 10-6 plates = TNTC 107 plate counted 134 colonies Overnight CFU/ml = E. coli 102, 104, 10-5 plates = TNTC 10-6 plate counted 173 colonies Overnight CFU/ml =
3) Your initial E.coli concentration is 1x10° CFU/ml (initial tube). The serial dilutions for each tube vou performed were as follows: 2 mls from initial tube into 198 mls of saline (tube 1), 5 ml from tube 1 into 5 mls of saline (tube 2). And then 0.1 ml from tube 2 into 0.9ml saline in tube 3. You plated 0.1ml on one plate from tube 3. Based on this scheme, how many colonies (CFU) do you expect on that plate? Draw out the dilution scheme described above and show me your answer and complete work. lorada la oi ain doo oiloo inon Viable colony counts of overnight (ON) cultures: Dilutions are total/serial plate dilutions after plating 0.1ml of solution from the indicated dilution tube. Serratia marcescens 102, 104, 10-6 plates = TNTC 107 plate counted 134 colonies Overnight CFU/ml = E. coli 102, 104, 10-5 plates = TNTC 10-6 plate counted 173 colonies Overnight CFU/ml =
Chapter14: Formula Method
Section: Chapter Questions
Problem 40SST
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