What color are Gram negative cells after decolorizing with ethanol?What is the best age for your culture when performing a Gram stain? Why?Why is Gram staining considered a differential staining process?What are the primary and counterstain?What is the purpose for using a counterstain?What are some reasons a Gram positive cell might appear Gram negative?What color would a Gram negative cell be if you forgot to wash with ethanol and why?What color would a Gram negative cell be if you forgot to stain it with safranin and why?

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What color are Gram negative cells after decolorizing with ethanol? What is the best age for your culture when performing a Gram stain? Why? Why is Gram staining considered a differential staining process?

What color are Gram negative cells after decolorizing with ethanol? What is the best age for your culture when performing a Gram stain? Why? Why is Gram staining considered a differential staining process?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

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Concept explainers

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.

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What color are Gram negative cells after decolorizing with ethanol?
What is the best age for your culture when performing a Gram stain? Why?
Why is Gram staining considered a differential staining process?
What are the primary and counterstain?
What is the purpose for using a counterstain?
What are some reasons a Gram positive cell might appear Gram negative?
What color would a Gram negative cell be if you forgot to wash with ethanol and why?
What color would a Gram negative cell be if you forgot to stain it with safranin and why?

Definition Definition Technique that uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.

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