An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present?   No acid-fast bacteria are present on the slide Methylene blue was not used Carbolfuchsin was not used Acid-alcohol was not used

Question

An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present?

 

  1. No acid-fast bacteria are present on the slide
  2. Methylene blue was not used
  3. Carbolfuchsin was not used
  4. Acid-alcohol was not used

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