If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would you expect? a) gram negative bacteria would stain purple b) gram negative bacteria would be unstained c) gram positive bacteria would stain pink d) gram negative and gram positive would be unstained

If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would you expect? a) gram negative bacteria would stain purple b) gram negative bacteria would be unstained c) gram positive bacteria would stain pink d) gram negative and gram positive would be unstained

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 2RQ

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Concept explainers

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.

Question

If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would you expect?

a) gram negative bacteria would stain purple

b) gram negative bacteria would be unstained

c) gram positive bacteria would stain pink

d) gram negative and gram positive would be unstained

Definition Definition Technique that uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.

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