Ladder LS KT MV DY JY Ladder 850 bp 550 bp 11 1

Q: For each individual on gel, determine if the individual is homozygous for presence of the Alu…

A: In gel electrophoresis the fragments which are Small os size ( less base pairs) travel further in…

Q: For each individual on each gel, determine if the individual is homozygous for presence of the Alu…

A: An Alu element is a short stretch of DNA originally characterized by the action of the Arthrobacter…

Q: Shown below are two homologous lengths of the alpha and betachains of human hemoglobin. Consult a…

A: the minimal mutational distance is

Q: determine if the individual is homozygous for presence of the Alu insertion(++), heterozygous (+-),…

A: Each Alu sub family member assertion locus was assayed by PCR analysis. It is done by using…

Q: A diploid organism with a 45,000-kb haploid genome contains 21% G residues. Calculate the number of…

A: Deoxyribonucleic acid is a molecule composed of two polynucleotide chains that coil around each…

Q: Calculate the dynamic programming matrix and the optimal local and global alignment for the DNA…

A: a: GAATTC b: GATTA Scoring:- +2 for a match -1 for a mismatch -2 L for linear gap

Q: What two basepair changes can occur when G tautomerizes to the enol form? Recall that the…

A: Introduction: Spontaneous mutations result from numerous sources such as errors during DNA…

Q: 5-Bromouracil is a compound that is known to induce point mutations in DNA. 1) Out of A/C/G/T as…

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: How many units (U) of EcoR1 did you use in the digestion? How much lambda DNA (in ugs) was digested…

A: DNA-cutting enzymes are restriction enzymes. Each enzyme recognises a single or a few target…

Q: Assume that a DNA sequencing reaction is carried out, except that the four different…

A: Ans: DNA sequencing: It is the process of knowing the sequence of the DNA. The DNA sequencing is of…

Q: That's the result of Gel electrophoresis of genomic DNA ( Of genomic DNA extraction experiment),…

A: Agarose gel electrophoresis is mainly used for nucleic acid separation due to its large pore size…

Q: When aligning the three sequences (ATGC, AATC, AATG), one can use a 3-D "Manhattan Grid", with each…

A: ANSWER: ATG-C AAT-C AA-TG

Q: What does the third position imply when it comes to tRNA wobble effect and what the wobble effect…

A: Wobble base pairing : It is a pairing between two nucleotides in RNA molecules . The four main…

Q: Compare the structural features that distinguish B-DNA from A-DNA and Z-DNA. What is known about the…

A: DNA is the genetic material in almost all organisms with the exception of RNA viruses. DNA exists in…

Q: Consider the following coding sequence transcribed from 5' to 3' 5' A T G A A G C G C T C A G T A 3'…

A: Substitution of guanine at position 11 would result in to conversion of UCA into UGA (which is a…

Q: In the Sanger (dideoxy) method for DNA sequencing, a small amount of a dideoxynucleoside…

A: Sanger sequencing, also termed chain -terminator sequencing process is based on the ability of the…

Q: The specificity of Watson-Crick nucleobase pairing enables the biological information to be encoded…

A: Adenine, guanine, thymine, and cytosine are the nitrogen bases in the DNA. Adenine, guanine, uracil,…

Q: 5'-GATTACAATAGGAATTC~~ GAATTCTCGTGACTCCG-3' 3'-CTAATGTTATCCTTAAG~ CTTAAGAGCACTGAGGC-5' Which of the…

A: PCR or polymerase chain reaction is a DNA amplifying technique where a short piece of a gene or a…

Q: What is the principle behind the very specific sequence in donning and doffing PPEs? Explain

A: PPE -- refers to the personal protective equipments which help against the spread of infection and…

Q: Given the partial transposons DNA sequence 5’-ACCGTATTCGGT-3’ upstream from the central region,…

A: Transposons are the regions in the DNA molecule that can change their position within the genome.…

Q: What would be a primer sequence synthesized from the following DNA sequence: 5'-ACGTG-3'?

A: In the polymerase chain reaction (PCR), a primer is a short, single-stranded DNA sequence. A pair of…

Q: What is the significance that the cleavage site is 10-base-pairs long?

A: Biomolecules are organic molecules that occur and function in living systems. They include…

Q: V3 amino acid sequence molecular clone 296 306 314 320 324 charge S1 phenotype** 168.1 CTRPNNNTRKS…

A: The charge on the peptide or protein is due to the presence of acidic or basic amino acids. Acidic…

Q: Oxytocin, a hormone peptide of nine amino acids, is widely used in obstetrics to induce uterine…

A: Oxytocin(OT): It is a 9-amino acid peptide that is primarily synthesized in the neurons of the…

Q: Rice genome contains 30% C on a molar basis. What are the mole percentages of A, G, and T?

A: In a double helical DNA, there are 4 nucleotides are found ATP, GTP, CTP, TTP. In this double…

Q: What is the Kozak Sequence?

A: A small amino acid sequence act as an initiation sequence and binds to the mRNA in order to start…

Q: how are okazaki fragments created?

A: An Okazaki fragment refers to DNA fragments that are formed during the DNA replication process.

Q: DNA isolated from the bacterial virus M13 con-tains 25% A, 33% T, 22% C, and 20% G. Do these…

A: M13 is a filamentous bacteriophage that belongs to the Ff phage group. Ff phages are made up of 6407…

Q: If the DNA duplex for the beta chain of haemoglobin represented by the sequence…

A: Given the template strand in transcription unit is 3'-TACCACGTGGACTGAGGACTCCTCTTCAGA-5' If the…

Q: What is Barchyury protein? What is the structural aspects of DNA binding of Barchyury?

A: In 1927 brachyury mutation was first described in mice by adezhda Alexandrovna…

Q: What is Kozak sequence ? who invented this ?

A: Introduction  The process of transcribing the mRNA into protein is called Translation. There are 3…

Q: How base tautomerization causes mutation?

A: Mutations are changes that occurs in the deoxyribonucleic acid (DNA) sequence, either due to…

Q: GTT, AT,GсT стс, Ат, GCс GTT, ATт, сст стс, АТА, СGT T G C CCG CTG, ATA, AC C стт, АТА , С GT GTC,…

A: A primer is a short nucleic acid sequence that provides a starting point for DNA synthesis. A PCR…

Q: Extrachromosomal DNA is critical to the antibiotic resistance found in microorganisms, how do these…

A: Extrachromosomal DNA means a DNA which is present independently of the main DNA or chromosome.…

Q: The genetic đistance between DNA sequences 1 and 2 is what value?

A: Genetic distance : Distance between loci are determined by the frequency with which recombination…

Q: What is the purpose of the dideoxyribonucleoside triphosphates in the dideoxy-sequencing reaction?

A: DNA sequencing is carried out using various methods. The majorly used method of DNA sequencing is…

Q: In reversible terminator sequencing, how would the sequencing process be affected if the…

A: The basic between sanger's sequencing and reversible terminator sequencing is that Sangar's based on…

Q: Are all the proteins separated properly using two-dimensional (2D) gel electrophoresis? Why or why…

A: Two dimensional gel electrophoresis is an analytical technique that uses two techniques i.e.…

Q: Approximately how many Okazaki fragments are synthesized in thereplication of the human genome?

A: Okazaki fragments are short sequences of DNA nucleotides, which is synthesized during replication…

Q: The specificity of Watson-Crick nucleobase pairing enables the biological information to be encoded…

A: The double helical structure of DNA was discovered by Watson and Crick through diffraction studies.…

Q: What volume of 4X loading buffer must be added to 21 micro L of DNA in a technique for DNA sample…

A: Electrophoresis is the technique of seperation of RNA DNA and protein molecules on the basis of…

Q: The human genome contains approximately 106 copies of an Alusequence, one of the best-studied…

A: Alu elements are highly repeated DNA sequences that can be categorized as short interspersed…

Q: What are Okazaki fragments and how they are formed?

A: As the name suggests, DNA replication is a process that creates two identical copies of the original…

Q: How many base pairs are changed in the sickle cell mutation?

A: in sickle-cell anemia, the gene for beta globin is mutated. As a result the mutated protein is…