4. You can carry out matings between an Hfr and F−strain by mixing the two cell types in a small patch ona plate and then replica plating to selective medium.This methodology was used to screen hundreds of different cells for a recombination-deficient recA− mutant. Why is this an assay for RecA function? Wouldyou be screening for a recA− mutation in the F− or Hfrstrain using this protocol? Explain.
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
4. You can carry out matings between an Hfr and F−
strain by mixing the two cell types in a small patch on
a plate and then replica plating to selective medium.
This methodology was used to screen hundreds of different cells for a recombination-deficient recA− mutant. Why is this an assay for RecA function? Would
you be screening for a recA− mutation in the F− or Hfr
strain using this protocol? Explain.
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