magine you are working in a lab that is developing a novelty rose that smells like a stinkhorn mushroom – called the “Grose.” The gene that produces the odorous protein has been isolated from stinkhorn and recombinant DNA containing this gene, a promoter, and a gene that confers resistance to the antibiotic ampicillin has already been produced. Explain two methods you could use to introduce the recombinant DNA into rose cells. (ie. transforming the rose cells)
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
magine you are working in a lab that is developing a novelty rose that smells like a stinkhorn mushroom – called the “Grose.” The gene that produces the odorous protein has been isolated from stinkhorn and recombinant DNA containing this gene, a promoter, and a gene that confers resistance to the antibiotic ampicillin has already been produced.
Explain two methods you could use to introduce the recombinant DNA into rose cells. (ie. transforming the rose cells)
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