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- Identification and quantification of expressed genes initially requires a. reverse transcription of mRNA to cDNA b. fragmentation of the whole genome c. ligation to adapters of known sequences d. ligation of a target gene to a vectorThe sequencing of entire genomes has made it possible to examine the level of gene expression in a particular cell or tissue by using oligonucleotide probes to assess the mRNA expression level from a particular gene. This is done most effectively through the use of what experimental technique?What is the regulatory region of the Trp operon that is involved in alternative transcription termination mechanism? Choose from the options below. Attenuator Promoter Leader sequence Repressor Operator
- The specific sequence component of the bacterial promoter located 10 base pairs upstream of the a bacterial gene start site is called theIn eukaryotes, preinitiation complex is initiated by binding of the following factor to the promoter region of the protein-coding genes TFIID TFIIE and TFIIH RNA Polymerase Tata Binding ProteinsDescribe the difference between a transcriptional fusion and a translational reporter gene fusion. What sequences should you include in each? What types of information can be garnered from their analyses? Please include annotated pictures of the reporter gene expression patterns.
- In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account?The promoter and terminator regions of genes are important in: a. coding for amino acids b. gene regulation c. structural support for the gene d. intron removal e. anticodon recognitionA mutation in which of the following would still allow RNA polymerase to bind to promoters but would prevent the formation of an open complex? Question 4 options: TFIIB Mediator TFIIH TFIID TBP
- Molecules in higher eukaryotes that are directly involved in gene silencing include all of the following except a. miRNA b. argonaute c. microprocessor d. glycosylaseExplain the difference between gene modification and gene addition.Are the following examples of gene modification or gene addition?A. A mouse model to study cystic fibrosisB. Introduction of a pesticide-resistance gene into corn using theT-DNA vector of A. tumefaciensIn selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account? Explain your answer.