he steps to replicate the identified spike protein are as follows: . Find the CDNA sequence of the spike and make primers accordingly. Make CDNA from the viral RNA using the primers and then amplify this CDNA. . Clone the amplified DNA into a bacterial expression vector. .Express the spike protein in bacterial expression system. ( If this is not working because bacteria doesn't ave post-translational modification mechanism, then clone the spike gene in a mammalian expression ector) Express and purify spike protein from the above expression vector system. This way you can identify and replicate the identified protein spike.

he steps to replicate the identified spike protein are as follows: . Find the CDNA sequence of the spike and make primers accordingly. Make CDNA from the viral RNA using the primers and then amplify this CDNA. . Clone the amplified DNA into a bacterial expression vector. .Express the spike protein in bacterial expression system. ( If this is not working because bacteria doesn't ave post-translational modification mechanism, then clone the spike gene in a mammalian expression ector) Express and purify spike protein from the above expression vector system. This way you can identify and replicate the identified protein spike.

Name: Please express the following in detail The steps to replicate the iden
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Description: Please express the following in detail The steps to replicate the identified spike protein are as follows:1. Find the CDNA sequence of the spike and make primers accordingly.2. Make CDNA from the viral RNA using the primers and then amplify this CDNA

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter9: Gene Expression And Gene Regulation

Section: Chapter Questions

Problem 10QP: The pre-mRNA transcript and protein made by several mutant genes were examined. The results are...

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