What can you say about relative gene expression for the gene of interest, normalized to that of the housekeeper, using these cycle threshold data? Assume amplification efficiency is 100%. Note that gene expression is a measure of MRNA, but is accomplished by first converting the mRNA to copyDNA (CDNA) using reverse transcriptase. Average Ct Treatment A Treatment B Gene of interest 17.5 18.5 Housekeeping gene 18 17 It is 2-fold more abundant in treatment A. It is 2-fold more abundant in treatment B.

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