Mutations in bacterial promoters may increase or decrease the rate of gene transcription. Promoter mutations that increase transcription are termed up-promoter mutations, and those that decrease transcription are termed down-promoter mutations. The sequence of the −10 site of the promoter for the lac operon is TATGTT (see Figure 14.5). Would you expect each of the following mutations to be an up- promoter or down-promoter mutation? A. TATGTT to TATATT B. TATGTT to TTTGTT C. TATGTT to TATGAT

Mutations in bacterial promoters may increase or decrease the rate of gene transcription. Promoter mutations that increase transcription are termed up-promoter mutations, and those that decrease transcription are termed down-promoter mutations. The sequence of the −10 site of the promoter for the lac operon is TATGTT (see Figure 14.5). Would you expect each of the following mutations to be an up- promoter or down-promoter mutation? A. TATGTT to TATATT B. TATGTT to TTTGTT C. TATGTT to TATGAT

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Description: Mutations in bacterial promoters may increase or decrease therate of gene transcription. Promoter mutations that increasetranscription are termed up-promoter mutations, and those thatdecrease transcription are termed down-promoter mutations.The seque

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter29: Transcription And The Regulation Of Gene Expression

Section: Chapter Questions

Problem 1P

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Six strains of E. coli (mutants 1–6) that had one of thefollowing mutations (i–vi) affecting the lac operonwere isolated.i. deletion of lacYii. ocmutationiii. missense mutation in lacZiv. inversion of the lac operon (but not an inversion ofthe lacI gene)v. superrepressor mutationvi. inversion of lacZ, Y, and A but not lacI, P, oa. Which of these mutations would prevent the strainfrom utilizing lactose?b. The entire lac operon (including the lacI gene andits promoter) from each of the six E. coli strainswas cloned into a plasmid vector containing an ampicillin resistance gene. Each recombinant plasmidwas transformed into each of the six strains to create partial diploids. In analysis of these strains, mutant 1 was found to carry a deletion of lacY, so thisstrain corresponds to mutation i in the list above.Which of the other types of mutations would be expected to complement mutant 1 in these partial diploids so as to allow lactose utilization?c. In part (b), each strain was plated on…
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3. Jacques Monod, one of the geneticists who won the NobelPrize in medicine for deciphering the regulatory control of thelac operon, famously stated:“All that is true for E. coli, is true for the elephant.” • In 2-3 sentences, provide a rationale for why Monod’sstatement accurately reflects fundamental shared featuresof gene regulatory control in common between prokaryotesand eukaryotes. • In an additional 2-3 sentences, provide a critical analysisthat describes in detail AT LEAST TWO of the keydifferences between prokaryotes and eukaryotes in howthey regulate their genes.
. In an effort to determine the location of an operator sitefor a negatively regulated gene, you have made a seriesof deletions within the regulatory region. The extent ofeach deletion is shown by the line underneath the sequence, and the resulting expression from the operon(i = inducible; c = constitutive; − = no expression) isalso indicated.... GGAT C T T AGCCGGCTAACATGATAAATATAA......C C T AGAATCGGCCGA TTGTA C T A TTT ATAT T ...1 i2 –3 c4 –5 ca. What can you conclude from these data about thelocation of the operator site?b. Why do you think deletions 2 and 4 show no expression of the gene?
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Good afternoon, Guidance with this question would be most appriciated. Thank you for your time. Polypeptide sequences are formed from 20 amino acids. What is the probability that a single point mutation in a gene will result in a different polypeptide sequence?
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32. In class, I compared how the Lac operon operates like a car's gas pedal and brakes. Describe this comparison further by discussing individual elements of the Lac operon and connecting them to function of a car's gas pedal and brakes.
Plz answer ASAP. I will thumb up You are studying the regulation of the lactose operon in Escherichia coli, by measuring expression of the lacZ gene (i.e production of beta-galactosidase).(a) You identify several loss-of-function mutations in which lacZ is never expressed, in the presence and absence of glucose and lactose. What components of the lac operon could be mutated to produce this phenotype? List all possibilities.
35. The genes shown are from the lac operon system of E.coli. The symbols a, b and c represent the repressor (I) gene, the operator (O) region, and the structural gene (Z) for β-galactosidase, although not in that order. Furthermore, the symbols written in the genotypes are not necessarily the actual sequence in the lac operon.
94In the arrested state (7-14 nucleotides) of elongation, ( ) is required to reactivate transcription. 95C. elegans has about 19,000 genes, a significant number of which are organized into operons. Yesorno 96Transcription activator proteins have at least two independently folding domains: 1. DNA binding domain and 2.